Method for automatically constructing stable electrocatalytic bacterial biofilm

A bacterial biofilm and automatic construction technology, applied in the direction of biochemical fuel cells, etc., can solve the problems of poor bearing capacity, low electrocatalytic efficiency, and low catalytic activity, and achieve the effects of improved catalytic activity, large catalytic current, and strong catalytic activity

Inactive Publication Date: 2014-10-08
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The electrocatalytic bacterial biofilm constructed by the potential adaptation method generally has the following shortcomings: (1) the limit working current that the electrode can reach is small, and the electrocatalytic efficiency is low; Poor endurance, when the working current passing through the electrode approaches or exceeds its limit current, the electrode will be rapidly over-polarized, and over-polarization will cause the anode potential to move positively to +1.2V in a short period of time (usually no more than 10 minut

Method used

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  • Method for automatically constructing stable electrocatalytic bacterial biofilm
  • Method for automatically constructing stable electrocatalytic bacterial biofilm
  • Method for automatically constructing stable electrocatalytic bacterial biofilm

Examples

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Embodiment 1

[0035] The present invention automatically constructs the method for stable electrocatalytic bacterial biofilm, comprises the following steps successively:

[0036] (1) Assemble the microbial electrochemical reactor, which includes an anode chamber equipped with an anode plate and a cathode chamber equipped with a cathode plate.

[0037] ⑵ if figure 1 , figure 2 Connect circuit components as shown.

[0038] (3) Pre-start the microbial anode, inject bacterial growth medium solution into the anode chamber of the microbial electrochemical reactor to immerse the anode, inject cathodic solution into the cathode chamber of the microbial electrochemical reactor to immerse the cathode, and then inoculate the anode chamber with The microbial source of bacteria, and then turn the selection switch QS1 to connect with the first selection end so that the anode passes through the fixed resistance R 0 It is electrically connected to the cathode to pre-start the microbial anode.

[0039]...

Embodiment 2

[0046] The present invention automatically constructs the method for stable electrocatalytic bacterial biofilm, comprises the following steps successively:

[0047] (1) Assemble the microbial electrochemical reactor, which includes an anode chamber equipped with an anode plate and a cathode chamber equipped with a cathode plate.

[0048] ⑵ if figure 1 , figure 2 Connect circuit components as shown.

[0049] (3) Pre-start the microbial anode, inject bacterial growth medium solution into the anode chamber of the microbial electrochemical reactor to immerse the anode, inject cathodic solution into the cathode chamber of the microbial electrochemical reactor to immerse the cathode, and then inoculate the anode chamber with Then turn the selection switch QS1 to connect with the first selection terminal so that the anode is electrically connected to the cathode through the fixed resistance R0, and the microbial anode is pre-started.

[0050] The raw material composition and weig...

Embodiment 3

[0057] The present invention automatically constructs the method for stable electrocatalytic bacterial biofilm, comprises the following steps successively:

[0058] (1) Assemble the microbial electrochemical reactor, which includes an anode chamber equipped with an anode plate and a cathode chamber equipped with a cathode plate.

[0059] ⑵ if figure 1 , figure 2 Connect circuit components as shown.

[0060] (3) Pre-start the microbial anode, inject bacterial growth medium solution into the anode chamber of the microbial electrochemical reactor to immerse the anode, inject cathodic solution into the cathode chamber of the microbial electrochemical reactor to immerse the cathode, and then inoculate the anode chamber with The microbial source of bacteria, and then turn the selection switch QS1 to connect with the first selection end so that the anode passes through the fixed resistance R 0 It is electrically connected to the cathode to pre-start the microbial anode.

[0061]...

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PUM

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Abstract

The invention relates to a method for automatically constructing a stable electrocatalytic bacterial biofilm. The method comprises the following steps: firstly assembling a microbe electrochemical reactor, and connecting circuits, wherein the first circuit is as follows: a fixed resistor is connected between an anode and a cathode in series, and the second circuit is as follows: an adjustable direct-current power supply, an ampere meter and a work contact of a circulating time relay are connected between the cathode and the anode; then injecting a bacteria growth medium solution into an anode chamber, inoculating a bacteria source, prestarting a microbe anode by switching on the first circuit, then controlling pulse current between the anode and the cathode to be gradually increased at preset velocity according to the potential change of the anode by switching on the adjustable direct-current power supply of the second circuit, wherein the pulse current is immediately lowered by 40%-60% when the potential of the anode is increased to a limit value and enters a new round of growth stimulation, and the electrocatalytic bacterial biofilm is completely constructed till the pulse current value reaches a preset current limit value and is stable at the preset current limit value for more than 10 hours. The electrocatalytic bacterial biofilm disclosed by the invention can be stably operated with the relatively high working current.

Description

technical field [0001] The invention relates to a method for automatically constructing a stable electrocatalytic bacterial biofilm, which belongs to the technical field of microbial electrochemical reactors. Background technique [0002] Microbial electrochemical reactors include microbial fuel cells, microbial electrolysis cells, and microbial electrochemical sensors. In microbial electrochemical reactors, the electrocatalytic activity and operational stability of the electrocatalytic bacterial biofilm attached to the electrode surface are the key components that determine the performance of the reactor. A stable and efficient electrocatalytic bacterial biofilm can improve the performance of microbial fuel cells and microorganisms. Energy efficiency of electrolytic cells, increased sensitivity of microbial electrochemical sensor signal responses. Electrocatalytic bacterial biofilm is a cell accumulation layer with a certain spatial structure formed by the cells and extrac...

Claims

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Application Information

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IPC IPC(8): H01M8/16
CPCY02E60/527H01M8/16Y02E60/50
Inventor 张恩仁汪霄刁国旺
Owner YANGZHOU UNIV
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