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Genetic transformation and regeneration method for eucalyptus

A technology of genetic transformation and eucalyptus, which is applied in the field of genetic transformation and regeneration of plants, can solve the problems of low transformation efficiency, narrow scope of application, and heavy workload, and achieve the effects of low cost, wide applicability, and short cycle

Inactive Publication Date: 2014-10-29
CHINA EUCALYPT RES CENT
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Problems solved by technology

To sum up, at home and abroad, genetic transformation has been carried out using different parts of various eucalyptus as materials, and related transgenic plants have been obtained. Shortcomings such as low efficiency, there is no successful example of using eucalyptus budding stakes as explants for genetic transformation

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  • Genetic transformation and regeneration method for eucalyptus

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Embodiment Construction

[0016] The present invention will be further described below in conjunction with specific embodiments. In a specific embodiment, the EC FAD gene is used to construct a recombinant plasmid for Agrobacterium-mediated verification of the effectiveness of the Eucalyptus genetic transformation and regeneration method provided by the present invention. The EC FAD gene is a fatty acid desaturase (Fatty Acid) from Eucalyptus red Desaturase (FAD) gene is an important regulatory gene for the conversion of saturated fatty acids (C16:0, C18:0) to polyunsaturated fatty acids (C18:2, C18:3). The above-mentioned genes are used to construct recombinant plasmids in the examples, but it does not mean that the application of the Eucalyptus genetic transformation and regeneration method of the present invention is limited to the above-mentioned EC FAD gene. It should be understood that the method of the present invention can be used for various types of Eucalyptus. GMO work. In the embodiment, w...

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Abstract

The invention discloses a genetic transformation and regeneration method for eucalyptus. The method comprises the following steps: firstly, preparing infectious agrobacterium carrying an exogenous gene; then, preparing a sprout pile of eucalyptus as an explant; then, co-culturing the sprout pile of eucalyptus and the agrobacterium carrying the exogenous gene for genetic transformation and regeneration; and finally, carrying out selective rooting culture and transplanting on the generated sprout bud to obtain a planed transformation plant. By using the sprout pile of a eucalyptus seedling as the explant which is co-cultured and converted with the agrobacterium carrying an the gene, the generated sprout bud is screened and cultured in a rooting culture medium, and the sprout bud rooted is transplanted to obtain the planed transformation plant. By adopting the method for transgenetic operation of eucalyptus, a positive transformation plant can be obtained within about 50 days. The method has the characteristics of short period, simple operation, low cost, wide applicability and the like. The invention provides a quick transgenetic method for molecular breeding and batched transgenetic operation of eucalyptus, thereby accelerating the progress of cultivation of novel varieties of eucalyptus.

Description

technical field [0001] The invention belongs to a method for genetic transformation and regeneration of plants, in particular to a method for genetic transformation and regeneration of eucalyptus. Background technique [0002] Eucalyptus originated in Australia and nearby islands. It is the general name of Eucalyptus in Myrtaceae (Myrtaceae). There are more than 800 species and 137 subspecies. - An excellent tree species. Eucalyptus is widely cultivated in tropical and subtropical regions due to its many types, fast growth, resistance to barrenness, good stem shape, and wide application. Currently, the area of ​​eucalyptus plantations has exceeded one-fifth of the world's plantation area. With the development of social economy, the contradiction between the supply and demand of wood is increasing day by day, and the demand for pulp is gradually increasing, resulting in a huge gap in wood supply. Therefore, it is very urgent to develop high-quality, fast-growing and high-yi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/84A01H5/00A01H4/00
Inventor 陈鸿鹏谢耀坚
Owner CHINA EUCALYPT RES CENT
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