Method for sorghum sudanense in-vitro induction of tracheids
A technology of Sudan grass and induction tube, applied in the field of plant cell engineering, can solve the problem of inability to learn from each other, and achieve the effect of high digestibility
Inactive Publication Date: 2014-11-05
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology
Because of the above differences, the influencing factors of in vitro induction of tracheid differentiation, such as the required nutrients, types and concentrations of hormones, and induction culture conditions are very different from those of sudan grass seeds and young ear tissue culture techniques. cannot learn from each other
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Embodiment 1
[0026] (1) Material
[0027] The Sudan grass inbred line 2098 was introduced from Japan in 1996; the wild germplasm of the pseudo-sorghum line was introduced from Fujian Agricultural College in 1998; the perennial Sudan was obtained in 2002 by distant hybridization with Sudan grass as the female parent and pseudo-sorghum as the male parent Grass (Kongzhi Gonggong, Zhong Xiaoxian, Gu Hongru, Ding Chenglong, etc. Preliminary report on the distant hybridization of Sudangrass and pseudosorghum. Acta Grassland Sinica, 2002, 10(1): 24-27.).
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The invention relates to a method for sorghum sudanense in-vitro induction of tracheid differentiation, and belongs to the technical field of plant cell engineering. The method comprises the steps: taking a light yellow granular callus tissue cultured and induced by a sorghum sudanense seed tissue as an explant, in a basic culture medium without plant growth hormones, adding 1.0-9.0 g / L of activated carbon, culturing for 5-21 d in dark and under scattered light for 16 h or 24 h daily and with the temperature of a culture room of 26-28 DEG C, carrying out in-vitro induction to obtain tracheids. The method for sorghum sudanense in-vitro induction of tracheid differentiation established by the invention has the tracheid differentiation rate reaching the highest value of 47.23%, and has the advantages that the method is simple and convenient in operation and high in induction efficiency, and provides a technical support for sorghum sudanense secondary cell wall lignin regulatory mechanism study and directional improvement.
Description
technical field [0001] The invention relates to a method for inducing tracheids of sudan grass in vitro, belonging to the technical field of plant cell engineering. technical background [0002] Sudan grass of the genus Sorghum is the most common gramineous forage grass cultivated in all countries in the world. It is eaten by all kinds of herbivorous livestock and poultry and herbivorous fish. As a main lignocellulosic high-energy-density crop, it has been paid more and more attention due to the characteristics of low cutting time and high photosynthetic efficiency. Studies have shown that for the production of the same amount of silage, the planting area of Sudan grass is 60% of that of silage corn, and the biogas production per unit weight of Sudan grass is 32% higher than that of silage corn. In Europe, 1 hectare of Sudan grass can be fermented to produce 2130 ~6060m 3 methane, while Sudan grass feed utilization and energy conversion efficiency were negatively correla...
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Inventor 钟小仙刘兆明刘智微马钟杰钱晨张建丽吴娟子
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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