Sperm brake fluid and preparation method thereof

A technology of sperm brake fluid and sperm, which is applied in the field of sperm brake fluid and its preparation, can solve the problems of high cost and poor performance of solution effectiveness, etc., and achieve strict detection and simple and reasonable preparation process operation , to avoid the effects of unknown factors

Inactive Publication Date: 2014-11-05
SHANDONG WEGO NEW LIFE MEDICAL DEVICES
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the sperm brake fluid in my country's assisted reproductive technology relies on imported finished products, the cost is high, and the effectiveness of the solu

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sperm brake fluid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0029] The preparation method of sperm brake fluid of the present invention comprises the following steps:

[0030] a. First weigh each component according to the proportion and place them separately;

[0031] b. Dissolve the weighed components (except bactericide, indicator, sodium bicarbonate, human serum albumin, polyvinylpyrrolidone PVP) in ultrapure water, follow the principle of solid before liquid during the dissolution process, NaHCO 3 the principle of last access;

[0032] c, add weighed bactericide, indicator and NaHCO in the solution obtained in step b 3 , dilute the solution to the required volume;

[0033] d. Detect the osmotic pressure and pH value of the solution. If the pH value is not within the range, adjust the pH value with prepared hydrochloric acid and sodium hydroxide solution, and record the final osmotic pressure and pH value to obtain the base liquid;

[0034] (3) Preparation of sperm brake fluid stock solution

[0035] a. Add PVP to the base liq...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a sperm brake fluid and a preparation method thereof. The sperm brake fluid contains a combination, a fungicide and an indicator which carry out treatment on sperms. Each index of the sperm brake fluid disclosed by the invention is in compliance with the standards of an in-vitro culture solution, the sperm brake fluid is safe in use, and whether the culture solution is deteriorated is easily judged, thereby providing a powerful guarantee for an assisted reproductive technology.

Description

technical field [0001] The invention relates to artificial assisted reproductive technology, in particular to a sperm brake fluid and a preparation method thereof. Background technique [0002] Sperm braking fluid has been widely used in IVF technology. The purpose of sperm brake fluid is to reduce sperm motility. The use of sperm braking fluid: a culture fluid that artificially reduces sperm motility when micromanipulating sperm. [0003] At present, the sperm brake fluid in my country's assisted reproductive technology relies on imported finished products, the cost is high, and the effectiveness of the solution cannot be well expressed. If the culture fluid is deteriorated and continues to be used, it will cause irreversible damage to experiments or clinical operations. the result of. Contents of the invention [0004] The purpose of the present invention is to provide a sperm brake fluid whose various indicators meet the standards of in vitro culture fluid, which is s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/076
Inventor 王维华吕汝举刘美静庄巍王英超
Owner SHANDONG WEGO NEW LIFE MEDICAL DEVICES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap