Application of prothymosin alpha to preparation of breast cancer diagnosis marker

Abstract

The invention relates to tumor markers, in particular to the application of prothymosin alpha to preparation of a breast cancer diagnosis marker. According to the application of prothymosin alpha to preparation of the breast cancer diagnosis marker, the dot blot hybridization method and the ELISA method are applied to detection of the level of prothymosin alpha protein in urine of a breast caner patient, and the relation between prothymosin alpha and the breast cancer biological behaviors is discussed; the immunoassay method is adopted, it is only required that the level of prothymosin alpha in urine is detected, it can quickly prove that the level of prothymosin alpha in urine of the breast cancer patient is obviously higher the normal level, preliminary judgment is conducted on breast cancer, and a basis is provided for further diagnosis; the needed urine sample is easy to obtain, any wound is avoided, an expensive instrument is not needed, operation is convenient, and popularization and application are easy.

Description

technical field [0001] The present invention relates to tumor markers, in particular to the application of prothymosin alpha in the preparation of breast cancer diagnostic markers. Background technique [0002] Breast cancer is one of the most common female malignancies, and the global incidence of breast cancer has been on the rise since the late 1970s [Yang L, Parkin DM, Ferlay J, et a1.Estimates of cancer incidence in China for 2000 and Projections for 2005 [J]. Cancer Epidemiol Biomarkers Prev, 2005, 14(1): 243-250]. One in eight women will suffer from breast cancer. China is not a country with a high incidence of breast cancer, but it should not be optimistic. This year, the growth rate of the incidence of breast cancer in my country is 1 to 2 percentage points higher than that of developed countries. According to the incidence data of breast cancer released by the National Cancer Center and the Bureau of Disease Control and Prevention of the Ministry of Health in 2012...

AI Technical Summary

Problems solved by technology

[0006] Prothymosin alpha is a strongly acidic protein widely expressed in vivo, and its function is related to cellular immune regulation, cell proliferation and apoptosis [Wu C L, Shiau A L, Lin C C. Prothymosin alpha promotes cell proliferation in NIH3T3 cells[ J].Life Sci,1997,61:2091-2099], as a typical tumor-associated protein, in-depth research on the regulation of cell proliferation and apoptosis helps to discover the mechanism of cell carcinogenesis. It has been used in various cancers The increase of PTMA level was detected in [Zheng Jinlai, Liu Dianxin, Gao Lanxing. Research progress of prothymosin α[J]. Foreign Medical Immunology Volume, 1998, 21(4):210-214]. The PTMA protein product not only maintains cells in a highly proliferative state, but also protects cells from entering the apoptotic pathway [EvatafievaαG, Belov C A, Rubtsov Y P, et al.Apoptosis-related fragmentation, translocation and properties of human prothymosinalpha[J].Exp Cell Res , 2003,284(2):211-223], it has been reported that the content of β group thymosin in breast cancer patients increases [Lan Haiyan et al. Expression and significance of thymosin β4, β10 and β15 in breast cancer[J] .Chinese Cancer Clinic and Rehabilitation, 2009,16(1):22-24], can be used as a postoperative recurrence marker, but the expression of thymosin pro-alpha in breast cancer is rarely reported [Xu Yali et al. Chinese female breast cancer incidence A case-control study on related risk factors[J]. Xiehe Medical Journal, 2011,2:7-14]

Images