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Method for constructing rat high-triglyceride acute pancreatitis model

A technology for acute pancreatitis and high triglycerides, which is applied in the field of animal model construction of diseases, and can solve problems such as inability to simulate high triglycerides pancreatitis

Inactive Publication Date: 2014-11-19
戴睿武
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for constructing a rat model of hypertriglyceride acute pancreatitis, which solves the problem that the prior art cannot simulate clinical hypertriglyceride pancreatitis

Method used

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  • Method for constructing rat high-triglyceride acute pancreatitis model
  • Method for constructing rat high-triglyceride acute pancreatitis model

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Experimental program
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Embodiment

[0020] A method for constructing a rat hypertriglyceride type acute pancreatitis model, comprising the following steps:

[0021] 1. Select 150-250g male SD rats;

[0022] 2. Inducing hyperlipidemia in rats: feeding with high-fat feed for 4 weeks;

[0023] 3. Surgical puncture:

[0024] (1) Inject pentobarbital sodium into the rats intraperitoneally to anesthetize them;

[0025] (2) Fix the rats on the operating board, shear the hair, routinely disinfect and spread the towel;

[0026] (3) Laparotomy was performed layer by layer along the linea alba, and the anterior mesenteric artery was exposed by blunt and sharp separation from the right side of the abdominal cavity of the rat;

[0027] (4) Carefully separate the anterior mesenteric artery from the surrounding tissue, suspend the two ends of the anterior mesenteric artery with two sterile silk threads, and place a sterile plastic sheet under the artery, gently pull the silk threads at both ends to lift the anterior mesente...

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Abstract

The invention discloses a method for constructing a rat high-triglyceride acute pancreatitis model, and solves the problem that high-triglyceride acute pancreatitis cannot be simulated clinically in the prior art. The method comprises the following steps: (1) rearing a male SD rat to have hyperlipemia; (2) injecting free fatty acid into the artery of the male SD rat, and inducing the acute pancreatitis through free fatty acid. According to the method, the occurrence of the high-triglyceride acute pancreatitis can be excellently simulated, and a good foundation is laid for research on the high-triglyceride acute pancreatitis.

Description

technical field [0001] The invention relates to a method for constructing an animal model of a disease, in particular to a method for constructing a rat high triglyceride type acute pancreatitis model. Background technique [0002] At present, the well-known method of establishing rat experimental acute pancreatitis model is to inject 5% sodium taurocholate retrogradely into the pancreaticobiliary duct to establish rat SAP model. This method is to retrogradely inject 5% taurocholate into the pancreaticobiliary duct of rats. Sodium 1.0ml / kg, injection speed 0.1ml / min, injection lasts 10 minutes. This method only simulates clinical pancreatitis caused by pancreatic duct obstruction and bile reflux, but cannot simulate the onset of high triglyceride pancreatitis. Contents of the invention [0003] The purpose of the present invention is to provide a method for constructing a rat model of hypertriglyceride acute pancreatitis, so as to solve the problem that the prior art cann...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/19A01K67/02A23K1/16A23K1/18A23K10/20A23K20/158A23K20/163A23K50/50
Inventor 戴睿武汤礼军陈光宇梁鸿寅邓超
Owner 戴睿武
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