Vaccine adjuvant as well as preparation method and application thereof

A technology of vaccine adjuvant and immune enhancer, which is applied in the field of its preparation and vaccine adjuvant, can solve the problems of insufficient vaccine stimulation, etc., and achieve the effects of enhancing immune stimulation ability, long stable period, and improving the efficacy of virus attack protection

Active Publication Date: 2014-11-19
HUAZHONG UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For the above defects or improvement needs of the prior art, the invention provides a vaccine adjuvant, its preparation method and application, the purpose of which is to make the oil-in-water nanoemulsion vaccine adjuvant enhance the vaccine itself by adding an immune enhancer. Cellular and humoral immune stim...

Method used

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  • Vaccine adjuvant as well as preparation method and application thereof
  • Vaccine adjuvant as well as preparation method and application thereof
  • Vaccine adjuvant as well as preparation method and application thereof

Examples

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preparation example Construction

[0051] Vaccine adjuvant provided by the invention, its preparation method, comprises the following steps:

[0052] (1) Uniformly disperse the immunopotentiator, the stabilizer and the complexing agent in the formula ratio in water to obtain the water phase.

[0053] (2) The oil phase and the emulsifier are mixed to obtain the oil phase containing the emulsifier.

[0054] (3) Slowly add the oil phase containing the emulsifier into the water phase and keep stirring, preferably magnetically stirring for 10 minutes to 40 minutes, so that a stable emulsion is formed.

[0055] (4) adjusting the pH value of the emulsion obtained in step (3) and constant volume to obtain colostrum. Preferably use 0.1mol / L sodium hydroxide solution to adjust the pH value.

[0056] (5) Perform high-speed shearing and high-pressure homogenization on the colostrum obtained in step (4), and filter and sterilize through a microporous membrane to obtain sterile nanoemulsion, which is the vaccine adjuvant. ...

Embodiment 1

[0061] The vaccine adjuvant provided by the invention is an oil-in-water nanoemulsion with a pH of 8.0. The vaccine adjuvant comprises 5% of oil phase, 5% of emulsifier, 1% of stabilizer, 3% of complexing agent and 0.5% of immune enhancer according to mass percentage, and the balance is water. The average particle size of the oil-in-water nanoemulsion is 108.4±17.6nm; the polydispersity index PDI is 0.174.

[0062] The immune booster is propolis with immune boosting effect.

[0063] The emulsifier includes a hydrophilic surfactant and a lipophilic surfactant, and the mass ratio of the hydrophilic surfactant to the lipophilic surfactant is 1.5:1. The lipophilic surfactant is mannide oleate, and its hydrophilic-lipophilic balance value is 4.3. The hydrophilic surfactant is Tween 80, and its hydrophilic-lipophilic balance value is 15.

[0064] The stabilizer is manganese gluconate.

[0065] The complexing agent is ethylenediaminetetraacetic acid.

[0066] The oil phase is Mo...

Embodiment 2

[0075] The vaccine adjuvant provided by the invention is an oil-in-water nanoemulsion with a pH of 8.5. The vaccine adjuvant comprises 10% of oil phase, 10% of emulsifier, 2% of stabilizer, 2% of complexing agent and 1% of immunopotentiator in terms of mass percentage, and the balance is water. The average particle size of the oil-in-water nanoemulsion is 745.2±20.8nm; the polydispersity index PDI is 0.207.

[0076] The immune enhancer is lentinan with immune enhancer effect.

[0077] The emulsifier includes a hydrophilic surfactant and a lipophilic surfactant, and the mass ratio of the hydrophilic surfactant to the lipophilic surfactant is 2:1. The lipophilic surfactant is polyoxyethylene sorbitol beeswax derivative. Its hydrophilic-lipophilic balance value is 3.0. The hydrophilic surfactant is polyoxyethylene castor oil, and its hydrophilic-lipophilic balance value is 13.3.

[0078] The stabilizer is manganese gluconate.

[0079] The complexing agent is ethylenediaminet...

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Abstract

The invention discloses an oil-in-water type vaccine adjuvant as well as a preparation method and application thereof. The oil-in-water type nano-emulsion vaccine adjuvant comprises the following components in percentage by mass: 0.1-10 percent of oil phase, 0.1-10 percent of emulsifier, 0.1-3 percent of stabilizer, 0.1-3 percent of complexing agent and 0.01-10 percent of immunopotentiator. The preparation method comprises the following steps: (1) uniformly dispersing the immunopotentiator, the stabilizer and the complexing agent in water, thereby obtaining an aqueous phase; (2) mixing an oil phase and an emulsifier, thereby obtaining an oil phase; (3) slowly adding the oil phase into the aqueous phase, and continuously stirring, thereby forming a stable emulsion; (4) regulating the pH value of the emulsion, and fixing the volume to obtain a primary emulsion; and (5) performing high-speed shearing and high-pressure homogenizing on the primary emulsion. The vaccine adjuvant provided by the invention is simple in preparation, convenient to use and small in side reactions, is used for diluting vaccines, particularly swine fever live vaccines and is high in stability and good in immune effect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and more specifically relates to a vaccine adjuvant, its preparation method and application. Background technique [0002] Adjuvants refer to substances that can promote, enhance or prolong the body's specific immune response to antigens after being added to vaccine preparations. Adjuvant is also an antigen adjuvant, which can significantly improve the immune effect of the vaccine when used together with the vaccine. Adjuvants have been used to enhance the immune effect of vaccines for more than 80 years. The immune adjuvant can assist the immunogen to stimulate the body to produce an earlier, stronger and longer-lasting immune response. The ideal immune adjuvant should meet the following conditions: non-toxic or minimal toxicity in the effective dose; can stimulate the body to produce a powerful humoral Immunity and / or cellular immune response; long-lasting immunity; no induction of autoimmunity; no...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K9/107A61K39/187A61P31/14
Inventor 刘卫梁孔贤许琦万江陵杨祥良
Owner HUAZHONG UNIV OF SCI & TECH
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