A kind of in vitro cell contact co-cultivation device and culturing operation method thereof

Abstract

The invention discloses an in-vitro cell contact type co-culture device and a culture operation method thereof. According to the device, a box bottom is arranged at the bottom end of a culture box body; a box cover is arranged at the upper end of the box body; a cell culture tank is arranged in the culture box body; the culture box body is in the shape of a conical gyro, and the diameter at the upper end of the culture box body is larger than the diameter of the bottom of the culture box body; the cell culture tank is barrel-shaped; the upper and lower diameters of the barrel are the same as the diameter of the box bottom of the culture box body; an isolating membrane is fixedly arranged in the middle of the culture tank, and divides the culture tank into an upper culture tank and a lower culture tank. The culture tank is ingeniously divided into the upper culture tank and the lower culture tank by using the polycarbonate membrane and is respectively inoculated on the upper and lower sides, and the culture tank is arranged in the cell culture box after being overturned, so that substantive cell contact type co-culture is realized, and influence of metabolites of one kinds of cells on another kinds of cells and an intercellular effect caused by direct contact are conveniently researched.

Description

technical field [0001] The invention relates to a cell culture device, specifically an in vitro cell contact type co-cultivation device and a culture operation method thereof. Background technique [0002] In animals, cells are in a highly informative microenvironment, in which there are various complex physical and chemical signals, and cells respond to various signal stimuli to achieve their specific biological functions. The interactions between cells and this microenvironment include a variety of complex biochemical, biomechanical, and bioelectrical signals from surrounding cells, the extracellular matrix, and soluble factors. Among them, cell-cell interactions occur through direct contact or paracrine soluble factors. In the late 1980s, in order to establish a culture system more similar to the in vivo environment, the in vitro environment and the in vivo environment should be matched as much as possible, so that cells can communicate with each other and support each o...

AI Technical Summary

Problems solved by technology

[0006] However, the existing cell co-culture devices cannot meet the needs of different forms and purposes of culture

In traditional contact co-culture, different cells are mixed together. It is difficult to control the interaction ratio between homotype and heterotype cells in the system, and it is also difficult to effectively separate different types of cells in later in-depth research, so it is difficult to detect two types of cells. Cell proliferation and differentiation and expression of related genes

Some cells are grown in the same cell culture dish or plate hole divided into different spaces. Since the cells are all grown on the bottom wall, in this case, only the exchange of secreted substances between different cells can be realized, but it cannot be achieved. substantive contact between

Just as the non-contact co-culture system can only partially simulate the interaction between cells in vivo, it lacks the intercellular interaction caused by direct contact between heterogeneous cells

Images