Application of DHFR in bladder cancer detection

A bladder cancer and bladder technology, applied in the application field of DHFR in the detection of bladder cancer, can solve the problems of little knowledge about bladder cancer, lack of systematic understanding of bladder cancer, etc.

Active Publication Date: 2014-11-26
深圳市飞点健康管理有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, we still lack a systematic understanding of somatic mutations in bladder cancer, and we know little about the key "driver genes" in bladder carcinogenesis

Method used

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  • Application of DHFR in bladder cancer detection
  • Application of DHFR in bladder cancer detection
  • Application of DHFR in bladder cancer detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Application of DHFR in the detection of bladder cancer:

[0025] 1. Sample source and selection criteria:

[0026] Tumor samples and matched peripheral blood or normal controls (adjacent morphologically normal bladder tissue) were obtained from newly tested patients through a Chinese member institution of the Genitourinary Cancer Genome Consortium (UCGC). According to the system stipulated by the institutional review committee, each patient signed an informed consent form before being recruited for the study. The detailed clinical data of the patients are shown in Table 1.

[0027] Table 1 Information of 99 patients with bladder cancer

[0028]

[0029]

[0030]

[0031] All specimens were snap-frozen in liquid nitrogen after collection and immediately stored at -80°C for further studies. Hematoxylin-eosin (HE) stained sections were independently evaluated under a microscope by two pathologists. In this study, we only selected tissues with a pu...

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Abstract

The invention relates to an application of DHFR in bladder cancer detection, and belongs to the technical field of molecular biology gene detection. The application includes the following steps: 1, respectively extracting DNA from the bladder tissue and peripheral blood of a sample to obtain DNA templates; 2, respectively carrying out real-time quantitative PCR amplification on the DNA extracted from the bladder tissue and peripheral blood of the sample with a primer pair P as a primer; and 3, calculating the log2 ratio of the sample according to delta delta Ct, and comparing the log2 ratio with copy number variation obtained by using whole genome sequencing data, and determining as a bladder cancer sample when the copy number variation is more than 2.5 or is less than 1.5 or when a G fraction obtained through an GISTIC algorithm is more than 0.08 or is less than 0.08. In the invention, sequence amplified DHFR can be used as a marker for the bladder cancer detection; and DHFR gene can be used as a gene target treatment kit of the bladder cancer.

Description

technical field [0001] The invention belongs to the technical field of molecular biology gene detection, and in particular relates to the application of DHFR in the detection of bladder cancer. Background technique [0002] Bladder cancer is the most common malignancy of the genitourinary system in various countries and regions around the world. An estimated 386,300 new cases and 150,200 deaths occurred in 2008 alone. Past studies have shown that bladder cancer is a highly heterogeneous disease with two different subtypes (superficial and invasive) with variable clinical manifestations and complex genetic background. Recently, we performed a study showing that eight chromatin remodeling genes (UTX, MLL-MLL3, CREBBP-EP300, NCOR1, ARID1A, and CHD6) were recurrently mutated in bladder transitional cell carcinoma. However, at present, we still lack a systematic understanding of the somatic mutations in bladder cancer, and we know little about the key "driver genes" in the proc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6851C12Q1/6886C12Q2600/118C12Q2600/156C12Q2531/113C12Q2537/16C12Q2561/113
Inventor 吴松黄毅杨坤蒋涛涛谢畅毛有胜蔡志明
Owner 深圳市飞点健康管理有限公司
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