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In-situ bladder cancer animal model and identification method

An animal model and identification method technology, which is applied in the fields of medical science and veterinary instruments to achieve the effect of high accuracy and sensitive method

Inactive Publication Date: 2014-12-03
XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Application Information

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Problems solved by technology

However, after the model is established, the pathological diagnosis can only be made by sacrificing the rats to judge whether the model is successfully established, so that the next step in vivo experiments cannot be carried out on the modeled animals.

Method used

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  • In-situ bladder cancer animal model and identification method
  • In-situ bladder cancer animal model and identification method
  • In-situ bladder cancer animal model and identification method

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Embodiment 1

[0041] 1. Experimental materials

[0042] 1. Laboratory animals

[0043] SD rat: SPF SD rat, female, 10-12 weeks old, weighing 200g±10g, purchased from Shanghai Experimental Animal Center of Chinese Academy of Sciences.

[0044] 2. Main reagents and materials

[0045] N-methylnitrosourea (MNU) powder: Sigma Company, USA

[0046] Sodium citrate powder: China National Pharmaceutical Group Shanghai Chemical Reagent Company, China

[0047] Pentobarbital powder: Serva, Germany

[0048] 3. Main instruments

[0049] AO8120 rotary paraffin microtome: Leica Microtomes, Germany

[0050] Leica Hi1210 spreader: Leica, Germany

[0051] Beckman Ф32 PH meter: Beckman Company, USA

[0052] Sartorius BP211D electronic balance: Sartorius, Germany

[0053] Nikon E600 optical microscope: Nikon Corporation, Japan

[0054] Nikon DXM 1200F high-definition color pathological imaging system: Nikon Corporation, Japan

[0055] Light speed 16-slice spiral CT machine: GE Company, USA

[0056] 2. Experimental method

[0057]...

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Abstract

The invention relates to an in-situ bladder cancer animal model. An establishment method of the animal model comprises the step of perfusing 20mg / mL carcinogen MNU solution in a bladder of an SD rat to cause the bladder cancer, wherein 0.1mL of carcinogen MNU solution is perfused per time per week, and six times in total. The in-situ bladder cancer animal model has the advantages that the novel establishment method is provided for the in-situ bladder cancer animal model; a novel method for establishing the model of detecting the bladder cancer through a living body is provided, so that a tumour model not beneficial to further in vivo experiment can be removed, and meanwhile, the effect of pretherapy and post-treatment of the bladder caner animal model can be evaluated by utilizing CT (Computed Tomography) scanning; the method for diagnosing the in-situ bladder cancer of the rat through CT scanning is more sensitive, higher in accuracy, and more direct and convenient, and can provide convenience for further experiment research of the animal model.

Description

technical field [0001] The invention relates to the field of animal models, in particular to an orthotopic bladder cancer animal model and an identification method. Background technique [0002] In my country, bladder tumors are the most common tumors in the urinary system, and the establishment of animal models of bladder cancer is of great significance for the study of human bladder cancer. Studies and reports at home and abroad have confirmed that N-methyl nitrosourea (MNU) can be used as a chemical carcinogen to establish orthotopic bladder cancer animal models. However, after the model is established, the pathological diagnosis can only be made by sacrificing the rats to judge whether the model is successfully established, so that the next step in vivo experiments cannot be carried out on the modeled animals. Contents of the invention [0003] The purpose of the present invention is to provide an orthotopic bladder cancer animal model aiming at the deficiencies in th...

Claims

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Application Information

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IPC IPC(8): A61D7/00
Inventor 潘春武齐隽
Owner XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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