Method for synchronously and efficiently preparing chuanminshen violaceum protein and polysaccharide
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A technology of Chuanming ginseng and Ming ginseng, which is applied in the field of synchronous ultrasonic-assisted extraction of protein and polysaccharides, can solve the problems of increased cost and low total protein, and achieve the effects of low production cost, simple operation, and short extraction time
Active Publication Date: 2014-12-03
SICHUAN AGRI UNIV
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[0003] At present, most of the polysaccharides and proteins of Chuanming ginseng are extracted separately, but because the total...
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Embodiment 1
[0046] Chuanming ginseng powder, with pH 12.5 alkaline solution as the extractant, was ultrasonically extracted under the conditions of ultrasonic power 225W, ultrasonic time 38min, liquid-to-solid ratio 20:1, and ultrasonic temperature 49°C. Filter the extract to adjust the pH to 2.0 to precipitate the protein, centrifuge, wash the precipitate at 300pa, and freeze-dry at -70°C to obtain the Chuan Mingshen protein; the supernatant is concentrated under reduced pressure to 1 / 4 of the original volume, and absolute ethanol is added to reach an alcohol content of 80%. Stand overnight at 4°C to precipitate the precipitate, centrifuge, wash the precipitate with ethanol three times, freeze-dry at 300 Pa, -70°C, and extract polysaccharides from Dechuan Mingshen base. The extraction rates of proteins and polysaccharides from Chuanmingshen were (2.42±0.84)% and (25.92±0.67)%, respectively.
[0047]
[0048]
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Abstract
The invention belongs to the technical field of food processing and particularly relates to a method for synchronously and efficiently extracting protein and polysaccharide from chuanminshen violaceum residues. The method comprises the following steps: after degreasing ethanol-extracted chuanminshen violaceum residues, adding an alkaline liquid with pH of 12.5 adjusted by sodium hydroxide according to a liquid and material ratio of 20 to 1; extracting twice under the condition of ultrasonic-assisted extraction, wherein the ultrasonic power is 225W, the ultrasonic time is 38 minutes and the ultrasonic temperature is 49 DEG C; combining the supernate; adjusting the pH to 2.0 by hydrochloric acid to precipitate protein; centrifuging to obtain the precipitate and supernate; after washing and neutralizing the precipitate, freeze-drying to obtain chuanminshen violaceum protein powder; and concentrating the supernate under reduced pressure, carrying out alcohol precipitation and centrifugation, washing the precipitate and freeze-drying to obtain chuanminshen violaceum alkaline extracted polysaccharide. By adopting the method provided by the invention, the extraction rates of chuanminshen violaceum protein and polysaccharide are respectively (2.42+/-0.84)% and (25.92+/-0.67)%. The method provided by the invention has the advantages of short extraction time, simple operation and low production cost.
Description
technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a method for synchronous ultrasonic-assisted extraction of protein and polysaccharides from Chuanming ginseng. Background technique [0002] Chuanmingshen, also known as Mingshen, Mingshashen, and Tumingshen, is the root of Umbelliferae plant Chuanmingshen genus "chuanminshen violaceum Sheh et Shan". ground medicinal materials. The main production areas are Sichuan (Qingbeijiang, Jintang, Jianyang, Cangxi, Weiyuan, Beichuan, Pingwu, Bazhong, Nanchuan), Hubei (Yichang, Dangyang) and other places. It has the effects of nourishing yin and invigorating the lungs, strengthening the spleen, etc. It is mainly used to treat febrile disease and yin deficiency, cough due to lung heat, spleen deficiency and lack of food, and weakness after illness. Chuanming ginseng contains polysaccharides, proteins, coumarins, terpenes, sterols, lipids, glycosides, free organic matte...
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