Kit for detecting nervous necrosis viruses of pacific codfish

Abstract

The invention discloses a kit for detecting nervous necrosis viruses of pacific codfish. The kit comprises an upstream primer and a downstream primer for a PCR reaction and is characterized in that the upstream primer sequence is shown as SEQ ID NO.1; the downstream primer sequence is shown as SEQ ID NO.2; and the plasmid standard substance of a nervous necrosis virus gene contains the sequence shown as SEQ ID NO.3. The kit for detecting the nervous necrosis viruses of the pacific codfish, disclosed by the invention, has the advantages of strong specificity, high sensitivity, good repeatability, wide detection linear range and the like. The kit disclosed by the invention can be widely applied to the artificial culture of the pacific codfish; and parent fish carrying no virus is selected to breed and sperms, eggs, fertilized eggs and fish larvae are monitored in real time to guarantee that virus amount carried in the parent fish, the sperms, the eggs, the fertilized eggs and the fish larvae is at a low level and avoid that lots of fries are dead due to virus outbreak, so that the bottleneck of the artificial breeding of the pacific codfish is overcome.

Description

technical field [0001] The invention relates to a kit for detecting fish viruses, in particular to a kit for detecting neuronecrosis virus in Pacific cod broodstock and larvae. Background technique [0002] Pacific cod is an important marine economic fish in northern my country. At present, due to overfishing and environmental impact, the number of Pacific cod in China's coastal waters continues to decrease. For this reason, people have carried out artificial breeding to Pacific cod, but it just will appear short-term massive death phenomenon in larvae stage. Although studies have shown that fish mainly rely on the protection of non-specific immunity (including maternal antibodies) in the early stages of development, if the specific immune system develops later, the immunity of juvenile fish may be relatively weak for a period of time, which cannot meet the needs of fish. The needs of the body have led to the outbreak of the virus carried by the fish body itself, so that a ...

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Problems solved by technology

For this reason, people have artificially raised Pacific cod, but it will die in a short period of time in the juvenile stage

Although studies have shown that fish mainly rely on the protection of non-specific immunity (including maternal antibodies) in the early stages of development, if the specific immune system develops later, the immunity of juvenile fish may be relatively weak for a period of time, which cannot meet the needs of fish. The needs of the body lead to the outbreak of the virus carried by the fish itself, so that a large number of fish fry die

However, so far, there have been no reports about Pacific cod carrying viruses and detection kits, and a large number of fry deaths have become a bottleneck restricting the artificial breeding of Pacific cod

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