Method for extracting araboxylan from astragalus medicine residues by using ionic liquid

A technology of arabinoxylan and ionic liquid, which is applied in the field of traditional Chinese medicine extraction, can solve problems such as the difficulty of separating and purifying arabinoxylan, achieve good social and economic benefits, avoid low yields of enzymatic methods, and avoid environmental pollution. Effect

Inactive Publication Date: 2014-12-17
SHANXI UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to overcome the existing difficulties in the separation and purification of arabinoxylan, and provide a method for extracting arabinoxylan from astragalus dregs by using ionic liquid under the premise of ensuring that the polysaccharide structure and activity are not affected. In order to realize the comprehensive utilization of Astragalus medicinal resources

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  • Method for extracting araboxylan from astragalus medicine residues by using ionic liquid
  • Method for extracting araboxylan from astragalus medicine residues by using ionic liquid

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Embodiment 1

[0019] 1) Extraction of arabinoxylan: the dried astragalus medicinal residues are crushed, passed through a 100-mesh sieve, and set aside; the above powder is added to porcine pancreatic α-amylase (Tris-HCl buffer solution, pH 7.0, 200U / mL) Solution (mass ratio of powder to porcine pancreatic α-amylase solution 1:10), shake overnight at 37°C. After removing the starch, centrifuge at 5000 rpm and discard the supernatant. The precipitate was washed with deionized water, centrifuged at 5000 rpm, and the supernatant was discarded to collect the precipitate. After repeated washing three times, dry it for later use. Add 5g of ionic liquid [Bmim]NO to 1g of the above-mentioned medicinal dregs powder after starch removal 3 (1-butyl-3-methylimidazole nitrate), magnetically stirred at 65°C for 36 hours. After stirring and cooling, add 200 mL of deionized water, let it stand and remove the precipitate, and recover the filtrate. Concentrate the filtrate to remove water, add 50 mL of t...

Embodiment 2

[0027] After the dried Radix Astragali dregs are crushed, pass through a 100-mesh sieve, and set aside; add the above powder into porcine pancreatic α-amylase (Tris-HCl buffer, PH 7.0, 200U / mL) solution (powder and porcine pancreatic α-amylase Solution mass ratio 1:20), shake overnight at 37°C. After removing the starch, centrifuge at 5000 rpm and discard the supernatant. The precipitate was washed with deionized water, centrifuged at 5000 rpm, and the supernatant was discarded to collect the precipitate. After repeated washing three times, dry it for later use. Add 50g of ionic liquid [Bmim]NO to 5g of the above-mentioned dregs powder after starch removal 3 (1-butyl-3-methylimidazole nitrate), magnetically stirred at 90°C for 18 hours. After stirring and cooling, add 600 mL of deionized water, let it stand and remove the precipitate, and recover the filtrate. Concentrate the filtrate to remove water, add 200mL tetrahydrofuran, and let it stand to collect the precipitate, ...

Embodiment 3

[0029] After the dried Radix Astragali dregs are crushed, pass through a 100-mesh sieve, and set aside; add the above powder into porcine pancreatic α-amylase (Tris-HCl buffer, PH 7.0, 200U / mL) solution (powder and porcine pancreatic α-amylase Solution mass ratio 1:15), shake overnight at 37°C. After removing the starch, centrifuge at 5000 rpm and discard the supernatant. The precipitate was washed with deionized water, centrifuged at 5000 rpm, and the supernatant was discarded to collect the precipitate. After repeated washing three times, dry it for later use. Add 40g of ionic liquid [Bmim]NO to 3g of the above-mentioned medicinal dregs powder after starch removal 3 (1-butyl-3-methylimidazole nitrate), magnetically stirred at 70°C for 36 hours. After stirring and cooling, add 400 mL of deionized water, let it stand and remove the precipitate, and recover the filtrate. Concentrate the filtrate to remove water, add 150mL tetrahydrofuran, and let it stand to collect the pre...

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Abstract

The invention discloses a method for extracting araboxylan from astragalus medicine residues by using ionic liquid. The method comprises the following steps: drying and crushing the astragalus medicine residues; removing starch in astragalus medicine residue powder by using amylase; mixing treated astragalus medicine residue powder and the ionic liquid 1-butyl-3-methylimidazole nitrate to extract araboxylan; performing cleaning and deproteinization on extracted araboxylan, and finally performing low-temperature freeze drying to obtain glycan powder. The method disclosed by the invention is simple and convenient and easy to operate, can overcome the technical defects of separation and purification of existing araboxylan, and achieves the comprehensive utilization of astragalus medicinal material resources.

Description

technical field [0001] The invention relates to a method for extracting traditional Chinese medicine, in particular to a method for extracting arabinoxylan from Radix Astragali dregs by using ionic liquid. Background technique [0002] With the continuous deepening of the industrialization and scale of traditional Chinese medicine production, taking Astragalus membranaceus as an example, the annual domestic consumption exceeds 1 million tons, and it is increasing year by year. There are 616 kinds of Chinese medicinal materials stipulated in the 2010 edition of the Chinese Pharmacopoeia. If a large number of Chinese medicinal materials are extracted and the remaining medicinal residues are discharged directly, it will pollute the environment. In addition, some unscrupulous traders reprocess the medicinal residues and sell them as medicinal materials in order to pursue profits, which seriously endangers people's health. In recent years, although traditional Chinese medicine d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/14
Inventor 李科秦雪梅郝霞高凡茸王桂臻
Owner SHANXI UNIV
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