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Preparation method of clinical mesenchymal stem cells

A stem cell, clinical technology, applied in the field of cell biology, can solve problems that do not meet clinical applications, and achieve the effects of avoiding disease or allergy risks, maintaining activity, and ensuring effectiveness

Inactive Publication Date: 2014-12-17
SHANGHAI HUAYAN STEM CELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, although all kinds of mesenchymal stem cell preparation methods disclosed at present can obtain mesenchymal stem cells, none of them meet the standards for clinical application. System monitoring to achieve clinical standard products of mesenchymal stem cells

Method used

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  • Preparation method of clinical mesenchymal stem cells
  • Preparation method of clinical mesenchymal stem cells
  • Preparation method of clinical mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] This embodiment provides a method for preparing clinical mesenchymal stem cells, which specifically includes the following steps:

[0033] Step 1. Screening and preservation of umbilical cords: screening and collecting umbilical cords of healthy full-term maternal volunteers, placing them in umbilical cord preservation solution at 4°C, and separating them within 24 hours; the umbilical cord preservation solution is a serum-free medium, without Contain antibiotics; the detection items of the screening include carrying virus, genetic family history, etc.; the viruses such as hepatitis B, hepatitis C, AIDS, syphilis, etc.;

[0034] At the same time, a tube of maternal blood was collected for re-examination. The re-examination items were viral immunological detection and genetic detection of hepatitis B, hepatitis C, AIDS, and syphilis.

[0035] Step 2. Separation of the umbilical cord: transfer the umbilical cord to a Petri dish and wash it with normal saline; remove the l...

Embodiment 2

[0042] This embodiment provides a method for preparing clinical mesenchymal stem cells, which specifically includes the following steps:

[0043] Step 1. Screening and preservation of umbilical cords: screening and collecting umbilical cords of healthy full-term maternal volunteers, placing them in umbilical cord preservation solution at 4°C, and separating them within 24 hours; the umbilical cord preservation solution is a serum-free medium, without Contain antibiotics; the detection items of the screening include carrying virus, genetic family history, etc.; the viruses such as hepatitis B, hepatitis C, AIDS, syphilis, etc.;

[0044] At the same time, a tube of maternal blood was collected for re-examination. The re-examination items were viral immunological detection and genetic detection of hepatitis B, hepatitis C, AIDS, and syphilis.

[0045] Step 2. Separation of the umbilical cord: transfer the umbilical cord to a Petri dish and wash it with normal saline; remove the l...

Embodiment 3

[0052] This embodiment provides a method for preparing clinical mesenchymal stem cells, which specifically includes the following steps:

[0053] Step 1. Screening and preservation of umbilical cords: screening and collecting umbilical cords of healthy full-term maternal volunteers, placing them in umbilical cord preservation solution at 4°C, and separating them within 24 hours; the umbilical cord preservation solution is a serum-free medium, without Contain antibiotics; the detection items of the screening include carrying virus, genetic family history, etc.; the viruses such as hepatitis B, hepatitis C, AIDS, syphilis, etc.;

[0054] At the same time, a tube of maternal blood was collected for re-examination. The re-examination items were viral immunological detection and genetic detection of hepatitis B, hepatitis C, AIDS, and syphilis.

[0055] Step 2. Separation of the umbilical cord: transfer the umbilical cord to a Petri dish and wash it with normal saline; remove the l...

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Abstract

The invention provides a preparation method of clinical mesenchymal stem cells. The method comprises screening puerperas before umbilical cord collection; taking healthy and mature volunteer puerperas as collecting targets; then performing separation, amplification and large-scale culture till the third generation, performing cryopreservation, and performing a series of quality detection on intermediate products generated during the separating process and the freezing process. When the frozen cells revive and are washed for standby, no obvious decrease of the viability occurs. The preparation method of the clinical mesenchymal stem cells aims at clinical, unified and systematic monitoring on the entire process from raw material collection to cell preparation accomplishment to achieve clinical standard product of the mesenchymal stem cells.

Description

technical field [0001] The invention belongs to the field of cell biology, and in particular relates to a method for preparing clinical mesenchymal stem cells. Background technique [0002] Stem cells (Stem Cells, SC) are a kind of pluripotent cells with self-renewing ability, which are primitive and unspecialized cells. Under certain conditions, it can differentiate into a variety of functional cells, and has the potential to regenerate various tissues and organs and the human body. Stem cells exist in all multicellular tissues, can divide into a variety of specialized cells through mitosis and differentiation, and can use self-renewal to provide more stem cells. Stem cells come from many sources, including umbilical cord, cord blood and bone marrow. [0003] The blood that remains in the placenta and umbilical cord after a baby is born is an important source of stem cells. Hematopoietic stem cells in umbilical cord blood can be used to treat a variety of blood system di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 董成友朱兵锐江晔谢天
Owner SHANGHAI HUAYAN STEM CELL SCI & TECH CO LTD
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