A kind of extraction method of copper algae polyphenol

Abstract

An extraction method for sargassum horneri polyphenol is characterized by comprising: A, taking sargassum horneri as a raw material, crushing sargassum horneri, and sieving by using a 25-35 mesh sieve, so as to obtain a uniform sargassum horneri powder; B, accurately weighing a certain amount of sargassum horneri powder, adding an organic solvent aqueous solution with the concentration of 40%-90% according to the material-solution ratio of 1:9-11 (w / v), and fully uniformly mixing and immersing; C, immersing for 10-15 min, and using a supersonic-wave cell crushing machine to crush for 5-10 min to perform extraction of polyphenol; D, performing centrifugal processing on the extraction liquid, and controlling the processing technology of 3500-4500 rpm and 4-6 min; E, repeatedly extracting for 4-6 times, and merging the supernatants; and F, performing 45 DEG C evaporation concentration on the supernatant by a rotary evaporator, and then performing vacuum freeze drying, so as to obtain the sargassum horneri polyphenol extractive. The extraction method overcomes long time, high temperature, low extraction rate and other adverse effects of a conventional method of using water to extract polyphenol, and is relatively simple and time-saving and low in cost, and the extracted sargassum horneri polyphenol product is high in content and relatively strong in cell antioxidation performance.

Description

technical field [0001] The invention relates to the technical field of marine biology, and relates to the extraction of natural products in brown algae, in particular to a method for extracting copper algae polyphenols. Background technique [0002] Copper algae is a large marine brown algae, belonging to the genus Sargassum, with high polyphenol content in brown algae. Fucoidan, also known as phlorotannin, is a class of polymers from brown algae with phloroglucinol as its structural unit, which has many unique biological activities and medicinal values. Brown algae polyphenols can degrade SOD and polysaccharides, especially Sargassum, their extracts quickly turn brown to brown, and the SOD isoenzyme bands also disappear. The hydroxyl group determines the chemical properties and biological activities of brown algae polyphenols. main functional group. Brown algae polyphenols have strong physiological activity and strong free radical scavenging activity, which can block the ...

AI Technical Summary

Problems solved by technology

[0003] The general production method of brown algae polyphenols is to use brown algae as raw material, using water extraction method, that is, first mix the brown algae powder with boiling distilled water, move it into a boiling water bath for extraction for 40-50 minutes, and immediately filter it while it is hot under reduced pressure after the extraction is completed. Extract 2-3 times, then dry and pulverize to obtain the product, this method has the disadvantages of long time, high temperature, low extraction rate, etc., which is not conducive to industrial production

There is also a Chinese invention with the patent number CN201010277902.1 "A Method for Extracting Brown Algae Polyphenols from Organisms", which uses the following steps to extract brown algae polyphenols: raw material pretreatment, leaching, cooling and washing, resin adsorption, washing Dehydration and freeze-drying, this method uses organic solvents to crudely extract brown algae polyphenols, but antioxidants need to be added, the leaching time takes 1-6 hours, and cooling takes 2-8 hours. This method has many procedures and takes a long time , high cost and low production efficiency