Application of trans-cassia twig acid in preparing drug for resisting bradyarrhythmia

[0058] Example 1: Effect of trans-cinnamonic acid on the heart rate of normal rats

[0059] Take 30 healthy rats, half male and half female, weighing 220±30g, and randomly divide them into: ①Cinnamic acid drug group, ②Blank group, ③Positive control group, 10 rats in each group, half male and half male. After one week of adaptive feeding in the experimental environment, the rats were generally in good condition, and gavage was started.

[0060] In the cinnamonic acid group, 50-250 mg/kg of trans-cinnamic acid was dissolved in 1-2-propanediol to prepare a saturated solution, which was administered by gavage continuously for 7 days. The positive control group was intraperitoneally injected with atropine 0.15 mg/kg;

[0061] 2 hours after the last gavage, the rats were weighed, and rats were anesthetized by intraperitoneal injection of 3% pentobarbital sodium 50 mg/kg. The supine position was fixed, and the needle electrodes were connected subcutaneously to the limbs. The electrocardiogram of the second lead of the rats was continuously monitored. The rat's heart rate was continuously recorded for 5 min.

[0062] Observation indicators: heart rate changes of rats before and after modeling.

[0063] Observation method Use the MedLab biological signal acquisition and processing system to detect the heart rate of rats, select "Experiment" - "Physiological Experiment" - "Animal ECG", magnification: 1000, upper limit frequency: 100, time constant: DC; record the experimental data;

[0064] Select "Online Measurement" - "ECG Measurement" to analyze the heart rate changes of rats.

[0065] Statistical processing: Input the rat heart rate data into the computer, and use SPSS19.0 Chinese version statistical software for statistical processing. data to Indicates that independent samples t-test and analysis of variance were used for comparison between groups; the results were statistically significant with P<0.01 or P<0.05. The results are shown in Table 1:

[0066] Table 1: Comparison of the heart rate of rats in the cinnamic acid group and the blank group

[0067]

[0068] t-test: △Comparison of heart rate between blank group and cinnamomilic acid drug control group: △P<0.01; ▲Comparison of heart rate between Atr control group and blank group: ▲P<0.01

[0069] Conclusion: Cinnamominic acid can increase the heart rate of normal rats. Atropine was used as the positive control group.

[0070] Example 2: Effect of trans-cinnamic acid on the heart rate of rats with acetylcholine-induced bradycardia

[0071] 30 healthy rats, half male and half female, weighing 220±30g, were randomly divided into: ① cinnamic acid group, ② acetylcholine model group, 10 rats in each group, half male and half male. After one week of adaptive feeding in the experimental environment, the rats were generally in good condition, and gavage was started.

[0072] In the cinnamonic acid group, 50-250 mg/kg of trans-cinnamic acid was dissolved in 1-2-propanediol to prepare a saturated solution, which was administered by gavage continuously for 7 days. The saline group was intragastrically administered for 7 days, and the positive control group was intraperitoneally injected with atropine 0.15 mg/kg;

[0073] 2 hours after the last gavage, the rats were weighed, and rats were anesthetized by intraperitoneal injection of 3% pentobarbital sodium 50 mg/kg. The supine position was fixed, and the needle electrodes were connected subcutaneously to the limbs. The electrocardiogram of the second lead of the rats was continuously monitored. Rats were injected with 0.1% acetylcholine chloride 2ml/kg into the tail vein to establish the model, and the heart rate of the rat was continuously recorded for 30 minutes, and the heart rate was recorded at 5 minutes, 10 minutes, 20 minutes and 30 minutes after the injection; observation index: the heart rate change of the rat before and after the model was established.

[0074] Observation method Use the MedLab biological signal acquisition and processing system to detect the heart rate of rats, select "Experiment" - "Physiological Experiment" - "Animal ECG", magnification: 1000, upper limit frequency: 100, time constant: DC; record the experimental data;

[0075] Select "Online Measurement" - "ECG Measurement" to analyze the heart rate changes of rats.

[0076] Statistical processing: Input the rat heart rate data into the computer, and use SPSS19.0 Chinese version statistical software for statistical processing. data to Indicates that independent samples t-test and analysis of variance were used for comparison between groups; the results were statistically significant with P<0.01 or P<0.05. The results are shown in Table 2:

[0077] Table 2: Comparison of heart rate before and after modeling in each group of acetylcholine model

[0078]

[0079] t-test: Comparison of heart rate decline between the 30min ach model group and the cinnamic acid group: ▲P<0.05

[0080] Conclusion: After successful modeling with acetylcholine, the decrease in heart rate between the cinnamonic acid group and the acetylcholine model group at 30 minutes was P<0.05, and the rate of heart rate decrease in the cinnamonic acid group was smaller than that in the model group after 30 minutes, and there was a statistical difference.

[0081] Trans-cinnamic acid has the effect of resisting the bradyarrhythmia of the acetylcholine rat model, and can increase the heart rate of the rat model. Example 3: Effect of trans-cinnamic acid on the heart rate of rats with bradycardia induced by verapamil

[0081] Trans-cinnamic acid has the effect of resisting the bradyarrhythmia of the acetylcholine rat model, and can increase the heart rate of the rat model. Example 3: Effect of trans-cinnamic acid on the heart rate of rats with bradycardia induced by verapamil

[0082] Take 30 healthy rats, half male and half female, weighing 220±30g, and randomly divide them into: ① cinnamic acid group, ② verapamil model group, ③ positive control group, 10 rats in each group, half male and half male. After one week of adaptive feeding in the experimental environment, the rats were generally in good condition, and gavage was started.

[0083] In the cinnamonic acid group, 50-250 mg/kg of trans-cinnamic acid was dissolved in 1-2-propanediol to prepare a saturated solution, which was administered by gavage continuously for 7 days. The equal-volume normal saline group was given continuous intragastric administration for 7 days, and the positive control group was intraperitoneally injected with atropine 0.15 mg/kg;

[0084] 2 hours after the last gavage, the rats were weighed, anesthetized by intraperitoneal injection of 3% pentobarbital sodium 50 mg/kg, fixed in the supine position, needle electrodes were connected subcutaneously to the limbs, and the electrocardiogram of lead II of the rats was continuously monitored. After the heart rate was stable, Rats were injected with verapamil injection 5 mg/kg into the tail vein to establish a model, and the heart rate was recorded at 3 minutes, 5 minutes, 10 minutes, 20 minutes and 30 minutes after the injection;

[0085] Observation indicators: heart rate changes of rats before and after modeling.

[0086] Observation method Use the MedLab biological signal acquisition and processing system to detect the heart rate of rats, select "Experiment" - "Physiological Experiment" - "Animal Electrocardiogram", magnification: 1000, upper limit frequency: 100, time constant: DC; record the experimental data;

[0087] Select "Online Measurement" - "ECG Measurement" to analyze the heart rate changes of rats.

[0088] Statistical processing: Input the rat heart rate data into the computer, and use SPSS19.0 Chinese version statistical software for statistical processing. data to Indicates that independent samples t-test and analysis of variance were used for comparison between groups; the results were statistically significant with P<0.01 or P<0.05. The results are shown in Table 3:

[0089] Table 3: Comparison of heart rate before and after modeling in each group of verapamil model

[0090]

[0091] t-test: Comparison of heart rate decline between the 30min ver model group and the cinnamonylic acid group: ▲P<0.05

[0092]Conclusion: After successful modeling with verapamil, the decrease in heart rate between the cinnamonic acid group and the acetylcholine model group at 30 minutes was P<0.05.

[0093] Trans-cinnamic acid has the effect of resisting the bradyarrhythmia of the verapamil rat model, and can increase the heart rate of the rat model.