Molecular marker gmssr18-24 closely linked to soybean rust resistance gene and its application

A technology for soybean resistance to rust and rust, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem of no disease-resistant varieties and control methods, accelerate the reproduction rate of pathogenic fungi, and reduce soybean yield losses. and other problems, to achieve the effect of convenient and rapid detection methods, speeding up the breeding process, and saving production costs
CN104293922BActive Publication Date: 2016-03-30INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Publication Date
2016-03-30

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Abstract

The invention discloses a molecular marker GmSSR18-24 closely linked with a soybean rust resistance gene and application thereof. A method for identification or assisted identification of soybean rust resistance comprises the following steps of: carrying out PCR amplification by respectively taking the genome DNA of the soybean to be identified and SX6907 as templates and a PCR primer pair which comprises two single stranded DNA as shown in a sequence 1 in a sequence table and a sequence 2 in the sequence table; detecting an amplified product through electrophoresis; confirming the disease resistance of the soybean to be identified according to a standard. According to the invention, a genetic group is established by hybridizing a high-rust resistance soybean SX6907 material and two non-rust resistance soybean materials, a rust resistance gene is positioned, the closely linked molecular marker is developed, and molecular marker-assisted selection is carried out, so that the selection efficiency can be improved, and the breeding progress can be accelerated.
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Description

technical field

[0001] The invention belongs to the technical field of soybean molecular biology and genetic breeding. More specifically, it relates to a molecular marker GmSSR18-24 closely linked with the soybean rust resistance gene locus and its application, and also relates to the application of the molecular marker in soybean rust resistance breeding. Background technique

[0002] Soybean is an important oil crop and food crop in the world, and it is also a source of raw materials for animal husbandry, aquaculture, medicine, light industry, chemical industry and other industries. Soybean originated in my country and spread to the West in the 18th century. After the Second World War, the world's soybean production developed rapidly, and at the same time brought about the rapid development of processing and utilization. On the basis of ancient oriental food processing, the deep processing of oil, protein and functional ingredients and its variety of end food, health foo...

Claims

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