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Molecular marker GmSSR18-40 in close linkage with soybean rust resistance gene locus as well as application thereof

A technology for soybean resistance to rust and rust, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of no disease-resistant varieties and control methods, and achieve convenient, fast, and cost-effective detection methods. cost, the effect of speeding up the breeding process

Active Publication Date: 2014-12-24
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2004, soybean rust officially landed in the continental United States and quickly spread to the north. Since there are no effective disease-resistant varieties and control methods, the annual loss is as high as 7 billion to 10 billion US dollars

Method used

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  • Molecular marker GmSSR18-40 in close linkage with soybean rust resistance gene locus as well as application thereof
  • Molecular marker GmSSR18-40 in close linkage with soybean rust resistance gene locus as well as application thereof
  • Molecular marker GmSSR18-40 in close linkage with soybean rust resistance gene locus as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Using the primer pair GmSSR18-40 to identify the F of Tianlong No. 1 × SX6907 2 Individual plant resistance to rust

[0036] 1. PCR reagents for identification or auxiliary identification of soybean rust resistance

[0037] The PCR reagent for identification or auxiliary identification of soybean rust resistance in this embodiment consists of PCR primer pair GmSSR18-40, 10×Taq buffer, dNTP mixture, MgCl 2 solution, Taq DNA polymerase and ddH 2 O composition.

[0038] Among them, the PCR primer pair GmSSR18-40 consists of two single-stranded DNAs, a forward primer and a reverse primer, and its sequence is as follows:

[0039] Forward primer: 5'-TTGACTTCTTTACAAACAAATGTTGA-3' (sequence 1 in the sequence listing),

[0040] Reverse primer: 5'-CCAAGTCTAACTTTTTCCCTCAAA-3' (sequence 2 in the sequence listing).

[0041] 2. Soybeans to be identified

[0042] F of Tianlong No. 1 × SX6907 2 The segregation population is obtained as follows: Tianlong No. 1 and SX6907...

Embodiment 2

[0108] Embodiment 2, the acquisition of primer pair GmSSR18-40

[0109] The segregating populations used were non-rust resistant soybean variety Zhongdou 40 (Zhang Cheng, introducing six nationally approved soybean varieties, Farm Consultant, 2012, 4:35-37) and rust resistant soybean SX6907 (Shan Zhihui et al., a soybean rust Screening and identification of new resistance sources, Chinese Journal of Oil Crops, 2012, 34: 188-192) hybridization, hybrid offspring self-crossing, namely F 2 A total of 116 individual plants were isolated from the population.

[0110] to F 2 The 116 individual plants were analyzed by SSR molecular markers to obtain genotype data, and the genetic map was constructed using Joinmap 3.0, combined with the identification data of rust resistance inoculation, and the rust resistance gene was mapped using WinQTL cartographer 2.5, and the soybean resistance to rust was mapped on soybean chromosome 18. The rust gene locus rpp.18-1 belongs to complete dominan...

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Abstract

The invention discloses a molecular marker GmSSR18-40 in close linkage with a soybean rust resistance gene locus as well as an application thereof. A provided identifying or auxiliary identifying method for soybean rust resistance comprises the following steps: a to-be-identified soybean and SX6907 genome DNA (deoxyribonucleic acid) are taken as a template, a PCR (polymerase chain reaction) primer pair formed by two single stranded DNAs represented in a sequence 1 and a sequence 2 in a sequence table is used for PCR amplification, an amplification product is detected through electrophoresis, and the disease resistance of the to-be-identified soybean is determined according to the standard. The high rust resistance soybean SX6907 material and two soybean materials without rust resistance are hybridized to construct a genetic group, the rust resistance gene is positioned, closely linked molecular marker is developed, and marker-assisted selection is performed, so that the selection efficiency can be increased, and the breeding process can be speeded up.

Description

technical field [0001] The invention belongs to the technical field of soybean molecular biology and genetic breeding. More specifically, it relates to a molecular marker GmSSR18-40 closely linked with the soybean rust resistance gene locus and its application, and also relates to the application of the molecular marker in soybean rust resistance breeding. Background technique [0002] Soybean is an important crop that originated in China and is widely cultivated in countries such as the United States, Brazil, Argentina, China and India. Soybean is rich in protein and fat and active ingredients beneficial to the human body such as soap II, isoflavones, phospholipids, etc. It is one of the main sources of vegetable protein and oil in the world. At present, my country's soybean planting area, total output and per unit yield rank fourth in the world, and its production level lags behind the world's soybean production. In 2012, China's soybean planting area was 6.85 million he...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 单志慧陈海峰杨中路邱德珍赵胜陈李淼张婵娟袁松丽张晓娟陈水莲万乔周新安
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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