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Molecular marker GmSSR18-24 closely linked with soybean rust resistance gene and application

A technology for soybean resistance to rust and rust, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem of no disease-resistant varieties and control methods, accelerate the reproduction rate of pathogenic fungi, and reduce soybean yield losses. and other problems, to achieve the effect of convenient and rapid detection methods, speeding up the breeding process, and saving production costs

Active Publication Date: 2015-01-21
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-temperature and high-humidity climate conditions will accelerate the propagation of pathogenic fungi and lead to the spread of rust. If effective control is not carried out, it will cause 40% loss of soybean yield
In 2004, soybean rust officially landed in the continental United States and quickly spread to the north. Since there are no effective disease-resistant varieties and control methods, the annual loss is as high as 7 billion to 10 billion US dollars

Method used

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  • Molecular marker GmSSR18-24 closely linked with soybean rust resistance gene and application
  • Molecular marker GmSSR18-24 closely linked with soybean rust resistance gene and application
  • Molecular marker GmSSR18-24 closely linked with soybean rust resistance gene and application

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1. Using the primer pair GmSSR18-24 to identify the F of Zhongdou 40×SX6907 2 Individual plant resistance to rust

[0034] 1. PCR reagents for identification or auxiliary identification of soybean rust resistance

[0035] The PCR reagents for identification or auxiliary identification of soybean rust resistance in this embodiment consist of PCR primer pair GmSSR18-24, 10×Taq buffer, dNTP mixture, MgCl 2 solution, Taq DNA polymerase and ddH 2 O composition.

[0036] Among them, the PCR primer pair GmSSR18-24 consists of two single-stranded DNAs, a forward primer and a reverse primer, and its sequence is as follows:

[0037] Forward primer: 5'-GAAGAGGGTCTTCAAAAATCAATC-3' (SEQ ID NO: 1),

[0038] Reverse primer: 5'-TTGTTAATCAGGATCTATAAGACATTG-3' (SEQ ID NO: 2).

[0039] 2. Soybeans to be identified

[0040] Zhongdou 40×SX6907 F 2 The segregation population is obtained as follows: Zhongdou 40 and SX6907 are crossed, and the hybrid offspring are self-crossed, wh...

Embodiment 2

[0107] Embodiment 2, the acquisition of primer pair GmSSR18-24

[0108] The segregating population used was a non-rust-resistant soybean variety Zhongdou 40 crossed with rust-resistant soybean SX6907 (Shan Zhihui et al., Screening and identification of a new source of resistance to soybean rust, Chinese Journal of Oil Crops, 2012, 34: 188-192), F 2 A total of 116 individual plants were isolated from the population.

[0109] to F 2 The 116 individual plants were analyzed by SSR molecular markers to obtain genotype data, and the genetic map was constructed using Joinmap 3.0, combined with the identification data of rust resistance inoculation, and the rust resistance gene was mapped using WinQTL cartographer 2.5, and the soybean resistance to rust was mapped on soybean chromosome 18. The rust gene locus rpp.18-1 belongs to complete dominant inheritance.

[0110] Wherein, DNA extraction, PCR amplification, and electrophoresis are the same as those in Example 1 above.

[0111]...

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Abstract

The invention discloses a molecular marker GmSSR18-24 closely linked with a soybean rust resistance gene and application thereof. A method for identification or assisted identification of soybean rust resistance comprises the following steps of: carrying out PCR amplification by respectively taking the genome DNA of the soybean to be identified and SX6907 as templates and a PCR primer pair which comprises two single stranded DNA as shown in a sequence 1 in a sequence table and a sequence 2 in the sequence table; detecting an amplified product through electrophoresis; confirming the disease resistance of the soybean to be identified according to a standard. According to the invention, a genetic group is established by hybridizing a high-rust resistance soybean SX6907 material and two non-rust resistance soybean materials, a rust resistance gene is positioned, the closely linked molecular marker is developed, and molecular marker-assisted selection is carried out, so that the selection efficiency can be improved, and the breeding progress can be accelerated.

Description

technical field [0001] The invention belongs to the technical field of soybean molecular biology and genetic breeding. More specifically, it relates to a molecular marker GmSSR18-24 closely linked with the soybean rust resistance gene locus and its application, and also relates to the application of the molecular marker in soybean rust resistance breeding. Background technique [0002] Soybean is an important oil crop and food crop in the world, and it is also a source of raw materials for animal husbandry, aquaculture, medicine, light industry, chemical industry and other industries. Soybean originated in my country and spread to the West in the 18th century. After the Second World War, the world's soybean production developed rapidly, and at the same time brought about the rapid development of processing and utilization. On the basis of ancient oriental food processing, the deep processing of oil, protein and functional ingredients and its variety of end food, health foo...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 陈海峰单志慧杨中路邱德珍赵胜陈李淼张婵娟袁松丽张晓娟陈水莲万乔周新安
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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