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Method for Determination of Pyrogallic Acid Content by High Performance Liquid Chromatography-Internal Standard Method

A pyrogallic acid and high performance liquid chromatography technology, which is applied in measurement devices, instruments, scientific instruments and other directions, can solve the problems of low accuracy and large uncertainty of test results, and achieves scientific methods, easy acquisition and preservation, Avoid the effects of instrument instability

Active Publication Date: 2015-11-18
WUFENG CHICHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of can overcome the big uncertainty of detection result in the existing pyrogallic acid detection technology, the deficiencies in aspects such as low accuracy, promptly provide a kind of high performance liquid chromatography internal standard quantitative detection technology, the method Simple, accurate and reliable results

Method used

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  • Method for Determination of Pyrogallic Acid Content by High Performance Liquid Chromatography-Internal Standard Method
  • Method for Determination of Pyrogallic Acid Content by High Performance Liquid Chromatography-Internal Standard Method
  • Method for Determination of Pyrogallic Acid Content by High Performance Liquid Chromatography-Internal Standard Method

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Embodiment 1

[0047] 1. With the instruments and reagents adopted in the technical content of the present invention.

[0048] 2. adopt the high performance liquid chromatography mobile phase operation condition that adopts in the technical content of the present invention.

[0049] 3. Prepare standard solution.

[0050] The present invention uses ellagic acid as an internal standard to measure the content of pyrogallic acid.

[0051] 3.1 Preparation of internal standard solution: Weigh 0.1g (accurate to 0.0002g) of ellagic acid in a 250mL volumetric flask, dissolve it with methanol and dilute to the mark, ultrasonicate for 10min to fully dissolve it before use.

[0052] 3.2 Standard solution of pyrogallic acid: 2.0mg / mL. Accurately weigh 0.200g (accurate to 0.0002g) of pyrogallic acid into a 100mL volumetric flask, dilute to the mark with internal standard solution, shake well, and make it fully dissolved before use.

[0053] 4. Determination of pyrogallic acid content

[0054] 4.1 Making...

Embodiment 2

[0059] According to the analysis method and test procedure described in Example 1, 3,4,5-trimethoxybenzoic acid was used instead of ellagic acid as an internal standard to determine the content of pyrogallic acid. Obtained pyrogallic acid HPLC with 3,4,5-trimethoxybenzoic acid as internal standard ( image 3 ). It can be seen from the chromatogram that choosing 3,4,5-trimethoxybenzoic acid as the internal standard cannot achieve a good separation of the analyte pyrogallic acid and the internal standard. Therefore, 3,4,5-trimethoxybenzoic acid cannot be selected as the internal standard substance according to the analysis method and test procedure described in the method of the present invention.

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Abstract

The invention discloses a method precisely detecting content of coking gallate by adopting ellagic acid as an internal standard substance and applying high performance liquid chromatography internal standard quantitative analysis. According to the invention, uncertainty of the detection caused by instability of instruments when an external standard method is adopted for quantitative analysis to detect the content of the coking gallic acid is avoided; uncertainty of the detection caused by inconsistent sensitivity of various substances under the same detection condition when an area normalization method is adopted as the quantitative analysis method is also avoided; the method is convenient, quick, and accurate and reliable in detecting result.

Description

technical field [0001] The invention relates to a new method for measuring pyrogallic acid by high-performance liquid chromatography using an internal standard method as a quantitative analysis method, and belongs to the technical field of chemical analysis methods. Background technique [0002] Pyrogallic acid is an intensively processed product of Gallic gall, an important forest specialty in my country. It is widely used in the manufacture of pharmaceuticals, pesticides, dye intermediates, photosensitive materials, and electronic chemicals. It is also an important chemical reagent. It is used in the determination of bismuth, Antimony, silver, mercury, etc. are used to absorb oxygen in gas analysis, and are also the best reagents for determining wastewater COD under strong acid conditions. China is the main producer of pyrogallic acid, and the products are mainly exported to Japan, the United States and Europe. [0003] The determination of pyrogallic acid in the Japanese ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 汪咏梅张亮亮徐曼陈笳鸿
Owner WUFENG CHICHENG BIOTECH
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