PCR-LDR detection kit for three athletic ability related genes

Abstract

The invention discloses a combined detection kit for simultaneously detecting three athletic ability related genes with an LDR method. The detection kit can be used for simultaneously detecting three hotspot mutations of the three athletic ability related genes within three hours. The kit comprises a PCR amplification reagent and an LDR reagent, wherein the PCR amplification reagent comprises a PCR buffer solution, a reaction mixture of MgCl2 and dNTPs, a Taq enzyme, ultrapure water, and a primer mixture for carrying out high-specificity amplification on the three hotspot mutations of the three genes, namely ACTN3, EPO and CKMM, and the LDR reagent comprises a PCR product, a 10* connection buffer solution, LDR probes and Tap DNA ligase. The combined detection kit comprises three pairs of PCR primers and the three groups of detection probes in total. According to the invention, the three genes are taken as detection targets, and the method is high in sensitivity; compared with a traditional sequencing technique, the kit is capable of obtaining gene types of the three key genes significantly related to athletic ability once, can used for performing high throughput screening on athletic ability, and has the advantages of economy, high efficiency, high accuracy rate and the like.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a PCR-LDR detection kit for three motor-related genes. Background technique [0002] With the completion of the Human Genome Project and the full development of the Post-Genome Project, the application of genetic engineering-led biotechnology in the field of sports has received widespread attention. The application of genetic engineering to athlete selection is the general trend, and it will fundamentally cause a revolution in the theory and methods of sports selection. Preliminary progress has been made in sports selection using the theory and methods of genetics. Multiple gene loci related to individual athletic ability have been discovered, and the application of genetic engineering in sports talent selection has broad and urgent prospects. At present, the relevant loci for genetic screening of more mature sports talents include: [0003] 1. ACTN3 gene [0004]...

AI Technical Summary

Problems solved by technology

[0011] Currently commonly used gene detection methods include: direct sequencing (DS), ligase detection reaction (LDR), restriction fragment length polymorphism (RFLP), and denaturing high-performance liquid chromatography. Chromatographic analysis (denaturing high performance liquid chromatography, DHPLC), quantitative PCR, among which direct sequencing using a DNA analyzer is the gold standard, but the above methods have problems such as high cost, low accuracy, cumbersome operation and poor repeatability

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