Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Short-chain dehydrogenase capable of hydrolyzing ribose and variety of fluorinated ribose

A short-chain dehydrogenase and ribose technology, applied in the field of enzyme engineering, can solve the problems of unsatisfactory tumor specificity, high cost, and environmental pollution

Inactive Publication Date: 2015-03-11
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It should have the following disadvantages: 1) fluorinated glucose is prepared by chemical synthesis, which is costly and pollutes the environment, requiring strict equipment operation and personnel familiar with the operation; 2) FDG, as an analogue of glucose, can be absorbed by the body Many tissue parts are metabolized and absorbed, resulting in unsatisfactory specificity for the detection of tumors, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Short-chain dehydrogenase capable of hydrolyzing ribose and variety of fluorinated ribose
  • Short-chain dehydrogenase capable of hydrolyzing ribose and variety of fluorinated ribose
  • Short-chain dehydrogenase capable of hydrolyzing ribose and variety of fluorinated ribose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0022] The invention provides a short-chain dehydrogenase FribC enzyme capable of hydrolyzing ribose and various fluorinated ribose, and simultaneously provides the application of the enzyme in the biotransformation of sugar and radioactive material labeling. The specific preparation process is as follows:

[0023] (1) Obtain the open reading frame (ORF) from a newly isolated terrestrial Streptomyces by using the method of genome mining, and translate it to obtain the protein sequence.

[0024] (2) After designing and optimizing the DNA coding sequence, it is synthesized by in vitro chemical methods,

[0025] (3) AGGAGGTAAAACAT is used as the ribose binding site (ribosome binding site, RBS),

[0026] (4) Add a Met-Ser-Tyr at the N-terminus ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to short-chain dehydrogenase FribC capable of hydrolyzing ribose and a variety of fluorinated ribose and application thereof. The short-chain dehydrogenase is characterized in that a coding gene of the dehydrogenase is a gene sequence shown in a sequence 1 or an amino acid sequence shown in a sequence 2; a substrate of the dehydrogenase is D-ribose or modified D-ribose, and the modified D-ribose comprises 5-fluoro-5-deoxy-D-ribose, 4-difluoromethyl-5-deoxy-D-ribose or 4-trifluoromethyl-5-deoxy-D-ribose. The FribC provided by the invention can be prepared into a biocatalyst and is applied to the biotransformation of a variety of ribose; the FribC can also be prepared into a radiotracer used for positron emission tomography (PET).

Description

technical field [0001] The invention belongs to the technical field of enzyme engineering, in particular to a short-chain dehydrogenase capable of hydrolyzing ribose and various fluororibose and its application. Background technique [0002] Ribose has a wide range of functions in the life process, and ribose is also an important industrial raw material. The dehydrogenation reaction of cyclic ribose into lactone, lactone can then be hydrolyzed to form a chain carboxylic acid structure, which is helpful for its subsequent application and transformation, and is of great significance in the preparation of industrial raw materials and pharmaceutical intermediates. Dehydrogenases that can act on non-phosphorylative ribose are very rare and have been studied very little. Previously, only the research team of Professor Bradley Moore of the Scripps Research Institute in the United States discovered a short-chain dehydrogenase encoded by the SalM gene in the synthesis pathway of the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N9/04C12N15/53C12P7/42A61K51/08
CPCA61K51/08C12N9/0016C12P7/42
Inventor 马龙刁爱坡
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com