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Short-chain dehydrogenase capable of hydrolyzing ribose and various fluororibose and its application

A short-chain dehydrogenase and ribose technology, applied in the field of enzyme engineering, can solve problems such as high cost, environmental pollution, and unsatisfactory tumor specificity

Inactive Publication Date: 2018-08-28
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It should have the following disadvantages: 1) fluorinated glucose is prepared by chemical synthesis, which is costly and pollutes the environment, requiring strict equipment operation and personnel familiar with the operation; 2) FDG, as an analogue of glucose, can be absorbed by the body Many tissue parts are metabolized and absorbed, resulting in unsatisfactory specificity for the detection of tumors, etc.

Method used

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  • Short-chain dehydrogenase capable of hydrolyzing ribose and various fluororibose and its application
  • Short-chain dehydrogenase capable of hydrolyzing ribose and various fluororibose and its application
  • Short-chain dehydrogenase capable of hydrolyzing ribose and various fluororibose and its application

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Embodiment Construction

[0021] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0022] The invention provides a short-chain dehydrogenase FribC enzyme capable of hydrolyzing ribose and various fluorinated ribose, and simultaneously provides the application of the enzyme in the biotransformation of sugar and radioactive material labeling. The specific preparation process is as follows:

[0023] (1) Obtain the open reading frame (open reading frame, ORF) from a newly isolated Streptomyces terrestriale by using the method of genome mining, and translate it to obtain the protein sequence.

[0024] (2) After designing and optimizing the DNA coding sequence, it is synthesized by in vitro chemical methods,

[0025] (3) AGGAGGTAAAACAT is used as the ribose binding site (ribosome binding site, RBS),

[0026] (4) Add a Met-Ser-T...

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Abstract

The invention relates to short-chain dehydrogenase FribC capable of hydrolyzing ribose and a variety of fluorinated ribose and application thereof. The short-chain dehydrogenase is characterized in that a coding gene of the dehydrogenase is a gene sequence shown in a sequence 1 or an amino acid sequence shown in a sequence 2; a substrate of the dehydrogenase is D-ribose or modified D-ribose, and the modified D-ribose comprises 5-fluoro-5-deoxy-D-ribose, 4-difluoromethyl-5-deoxy-D-ribose or 4-trifluoromethyl-5-deoxy-D-ribose. The FribC provided by the invention can be prepared into a biocatalyst and is applied to the biotransformation of a variety of ribose; the FribC can also be prepared into a radiotracer used for positron emission tomography (PET).

Description

technical field [0001] The invention belongs to the technical field of enzyme engineering, in particular to a short-chain dehydrogenase capable of hydrolyzing ribose and various fluororibose and its application. Background technique [0002] Ribose has a wide range of functions in the life process, and ribose is also an important industrial raw material. The dehydrogenation reaction of cyclic ribose into lactone, lactone can then be hydrolyzed to form a chain carboxylic acid structure, which is helpful for its subsequent application and transformation, and is of great significance in the preparation of industrial raw materials and pharmaceutical intermediates. Dehydrogenases that can act on non-phosphorylative ribose are very rare and have been studied very little. Previously, only the research team of Professor Bradley Moore of the Scripps Research Institute in the United States discovered a short-chain dehydrogenase encoded by the SalM gene in the synthesis pathway of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/04C12N15/53C12P7/42A61K51/08
CPCA61K51/08C12N9/0016C12P7/42
Inventor 马龙刁爱坡
Owner TIANJIN UNIV OF SCI & TECH
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