Rapid protein analysis and detection device based on whole microfluidic chip closing system

Abstract

The invention relates to a micro device for performing on-line enrichment, elution, fluorescence labeling and detection on protein, and in particular provides a rapid protein analysis and detection device based on whole microfluidic chip closing system. The device is composed of a micro fluidic controlling system, a miniature laser induced fluorescence detection system, a microfluidic chip, an injection valve, a connecting pipeline, an interface and a data acquisition and processing part. A protein sample is enriched by an enriching column in a chip passage, is subjected to on-line fluorescence labeling operation and elution operation at the same time, and is induced by laser to be subjected to fluorescence detection. The rapid protein analysis and detection device has the advantages that the sample pre-processing process, fluorescence labeling and detection are integrated so as to enable cumbersome steps of an off-line analysis method to be omitted, the sample processing time is greatly shortened and the high sensitivity detection is achieved; the whole device is small in size and low in energy consumption; the microfluidic chip and a micro injection pump are connected through the interface with a special design; a whole fluid system runs in the closing system, the detection requirement under a special condition (such as non-gravity state) can be met. Thereby, the rapid protein analysis and detection device has the very good practical application value.

Description

technical field [0001] The invention relates to a protein detection device, and in particular provides a rapid protein analysis and detection device based on a microfluidic chip. Background technique [0002] Micro Total Analysis Systems (μ-TAS) is a new interdisciplinary field, the ultimate goal of which is to maximize the transfer of analytical laboratory functions to portable analytical instruments through the miniaturization and integration of chemical equipment. In the equipment (such as various types of chips), the "miniaturization" of the analysis equipment is realized. Its characteristic is that it can realize the sampling, dilution, addition of reagents, enrichment, extraction, mixing, reaction, separation, detection, etc. of the target analyte. Integration of steps. [0003] There are trace amounts of protein in body fluid, which is one of the important indicators to reveal the health of the human body, and is very important for evaluating its health status. Curr...

AI Technical Summary

Problems solved by technology

At present, commonly used detection methods such as chemical test paper method, ELISA, SDS-PAGE and other methods have problems such as poor accuracy or complicated operation. It is of great significance to develop a fast, simple and accurate detection method for protein in body fluids.

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