A kind of method for preparing sterile oil palm seed embryo samples in batches

Abstract

The invention discloses a method for preparing sterile oil palm seed embryo samples in batches. Use pruning shears to cut two-thirds of the mature oil palm fruit stalk centerline, and pick out the seed embryos with a dissecting needle; wash the robust and plump seed embryos with 0.01‑0.05% mercuric chloride Then soak and sterilize with 0.01-0.05% mercuric chloride in the ultra-clean workbench for 5-30min; after the sterilized seed embryos are rinsed with sterilized water, use sterilized filter paper to remove the sterilized water, and inoculate into the vitamin-containing MS of PhytoTechnology Company In the basic medium, culture in the dark at 20-30°C for 2-5 days, and then transfer to light culture conditions for 15-25 days to germinate. The method can obtain a large number of sterile oil palm seed embryo samples in a short period of time, has a low pollution rate and a high germination rate, and can meet the demand for a large number of samples in tissue culture, transcriptome, proteome, and other related research based on embryo culture.

Description

technical field [0001] The invention relates to the preparation of oil palm seed embryo samples, in particular to a method for preparing sterile oil palm seed embryo samples in batches. Background technique [0002] Oil palm is an important tropical oil-producing plant. At present, seedlings are mainly propagated by seeds, but there are problems such as long seed germination cycle and low germination rate. It is an important way to remove the physical constraints of highly lignified seed shells and peripheral dense endosperms, and then carry out research on seedling breeding or seed germination through embryo culture, and obtaining complete sterile seed embryos is a prerequisite. The oil palm embryo is in the shape of a long cone, about 2-3mm in length, soft and brittle, wrapped in a hard and dense endosperm, surrounded by highly lignified seed shells and oil-rich pulp. The existing technology usually needs to be sterilized with endosperm, and then the embryos are peeled in...

AI Technical Summary

Problems solved by technology

When peeling embryos with endosperm, it is difficult to grasp the force point, especially in the ultra-clean workbench with limited operating space, it is very easy to cause subsequent contamination or normal germination due to mechanical damage during the manual embryo removal process, which seriously slows down the extraction process. Embryo speed and inoculation speed