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Novel method for improving tyrosinase-based catalytic degradation of phenol pollutant

A technology of tyrosinase and catalytic degradation, which is applied in the field of degradation of phenol pollutants, and achieves the effect of high efficiency and simple equipment

Inactive Publication Date: 2015-03-25
CHINESE ACAD OF FISHERY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although ultrasonic degradation and photocatalytic degradation of zeolite-loaded antimony dioxide can achieve better degradation efficiency, they require external light sources and ultrasonic conditions, which have certain application limitations.

Method used

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  • Novel method for improving tyrosinase-based catalytic degradation of phenol pollutant
  • Novel method for improving tyrosinase-based catalytic degradation of phenol pollutant
  • Novel method for improving tyrosinase-based catalytic degradation of phenol pollutant

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Embodiment

[0047] figure 2 The dotted line in the middle represents the ultraviolet-visible spectrum of catechol degraded by tyrosinase; the solid line represents the synergistic degradation of phenol by 1-ethyl-3-methylimidazolium alanine ionic liquid and tyrosinase The ultraviolet-visible spectrum of catechol, the reaction time from bottom to top is 0, 4, 8, 12, 16, 20 minutes;

[0048] image 3 Curve a in the middle represents the time-absorption intensity diagram of catechol degraded by tyrosinase, and curve b represents 1-ethyl-3-methylimidazolium alanine ionic liquid and tyrosinase synergistically catalyze the degradation of phenol to generate Time-absorption intensity plot of catechol.

[0049] 1. Preparation of 1-ethyl-3-methylimidazole-alanine ionic liquid

[0050] 1) First, take equimolar ratio of bromoethane and nitrogen methyl imidazole into a round bottom flask, react at 50°C for 3 hours, then evaporate and remove the water in a vacuum, and prepare the ionic liquid inter...

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Abstract

The invention discloses a novel method for improving tyrosinase-based catalytic degradation of a phenol pollutant and relates to the technical field of phenol pollutant degradation. The novel method comprises the following steps that tyrosinase and 1-ethyl-3-methylimidazole alanine ionic liquid are mixed according to a ratio of 1: 80 to form a mixed solution, the mixed solution is added with a high-concentration phenol compound solution, a high-concentration phenol compound solution is added into tyrosinase with a concentration the same as that of tyrosinase used in the step a, experiment results of a 1-ethyl-3-methylimidazole alanine ionic liquid use group and a blank group are compared so that an o-dihydroxybenzene generation rate is obtained. The novel method has easy processes, is environmentally friendly, produces pollution-less catalytic degradation products and is economic and efficient.

Description

Technical field: [0001] The invention relates to the technical field of degradation of phenol pollutants, in particular to a novel method for enhancing tyrosinase catalytic degradation of phenol pollutants. Background technique: [0002] Phenol was discovered by the German chemist Longe in coal tar in 1834, so it is also known as carbolic acid (Carbolic acid). It is the simplest phenolic organic compound and a weak acid. It has a special odor and is extremely corrosive, and its concentrated solution has a strong stimulating effect on the skin. And during World War II, phenol was also used in Nazi concentration camps to execute prisoners. And phenol has a strong corrosive effect on the skin and mucous membranes, can inhibit the central nervous system or damage the liver and kidney functions. Acute phenol poisoning, that is, the absorption of high-concentration phenol vapor can cause symptoms such as headache, dizziness, fatigue, blurred vision, pulmonary edema, and acute re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34B01J31/04
Inventor 吴立冬宋怿
Owner CHINESE ACAD OF FISHERY SCI
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