Method for simultaneously extracting phycoerythrin and sulphated porphyra polysaccharide from inferior nori

A technology of phycoerythrin and sulfated polysaccharides, which is applied in the preparation method of peptides, chemical instruments and methods, algae/bryopeptides, etc., can solve the problems of pollution, low spectral activity of phycobiliproteins, complex components, etc., and reduce protein pollution , good polysaccharide uniformity and simple operation process

Inactive Publication Date: 2015-03-25
南通中国科学院海洋研究所海洋科学与技术研究发展中心 +1
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Problems solved by technology

However, these extraction processes all use dried laver powder, and the spectral activity of the phycobiliprotein obtained is very low, and the polysaccharide will inevitably be polluted by other molecules such as protein during the extraction process, and the composition is complex.

Method used

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  • Method for simultaneously extracting phycoerythrin and sulphated porphyra polysaccharide from inferior nori
  • Method for simultaneously extracting phycoerythrin and sulphated porphyra polysaccharide from inferior nori
  • Method for simultaneously extracting phycoerythrin and sulphated porphyra polysaccharide from inferior nori

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Effect test

Embodiment 1

[0042]Embodiment 1, the extraction and purification method of phycoerythrin

[0043] 1. Weigh 100g wet weight of fresh inferior laver and chop it up, add 1% phosphate buffer saline 10 times the weight of the algal body, overnight at 4°C to swell the cells.

[0044] 2. Centrifuge for 10 minutes at a rotational speed of 9000g and a temperature of 5-10°C, collect all the supernatant, and obtain 340ml of crude phycoerythrin extract. All residues were collected and stored in a -20°C refrigerator for extraction of sulfated polysaccharides.

[0045] The OD value of the crude extract of phycoerythrin was measured, and then according to the formula R-PE=155.8OD498.5-40.0OD614-10.5OD651, the crude extract contained 53.4mg of phycoerythrin, and the yield was 5.34mg / g.

[0046] figure 1 It is the absorption spectrum at 250-800nm ​​of the crude extract of Porphyra phycoerythrin.

[0047] 3.Pheny-Sepharose expanded column extraction. First equilibrate the Pheny-Sepharose expanded column...

Embodiment 2

[0062] Embodiment 2, the extraction of sulfated polysaccharide

[0063] Put the residue obtained after extracting the crude phycoerythrin extract into a conical flask, add 10 times the volume of 1% phosphate buffer with a pH of 6.0, control the temperature at 110-121°C for 1 hour, and then 8 layers of gauze Filtration; add 5 times the volume of 1% phosphate buffer solution with pH 6.0 to the filter residue again, and cook for another 1 hour at the same temperature; filter and combine the two filtrates to obtain the crude polysaccharide extract; rotate the crude polysaccharide extract Evaporate to a quarter of the original volume; add 4 times the volume of alcohol, collect the precipitate, and freeze-dry. The results are shown in Table 3.

[0064] Table 3 Yield and sulfate group content of Porphyra polysaccharides under different extraction conditions

[0065]

[0066] It can be seen that 3.29 grams of sulfated polysaccharides can be obtained with a yield of 3.29% by using...

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Abstract

The invention provides a method for simultaneously extracting phycoerythrin and sulphated porphyra polysaccharide from inferior nori. The method comprises the following steps: firstly, extracting phycoerythrin from fresh inferior nori; then, extracting sulphated porphyra polysaccharide from residues of phycoerythrin; and simultaneously extracting phycoerythrin and sulphated porphyra polysaccharide in a primary extraction process. The method provided by the invention can be used for obtaining phycoerythrin and sulphated porphyra polysaccharide in primary extraction. The obtained phycoerythrin is high in purity and the sulphated porphyra polysaccharide is good in uniformity. According to the method provided by the invention, the operation is simple and convenient, the process is simple, the separating speed is high, the cost is low, the utilization efficiency of inferior nori is improved, and resources are repeatedly used and utilized for many times.

Description

technical field [0001] The invention relates to the extraction and separation of algae functional substances, in particular to a method for simultaneously extracting phycoerythrin and laver sulfated polysaccharide from laver. Background technique [0002] Porphyra is a class of macroalgae with important economic value, and several species are currently widely cultivated in coastal countries of Southeast Asia. According to statistics, the annual output of laver in 2006 was about 1.8×10 4 tons of dry product, with an estimated annual output value of about US$1.3 billion (FAO, 2006). my country's laver industry is developing rapidly. From the point of view of distribution area, altar laver is the main one in the south of the Yangtze River, mainly concentrated in Fujian and Guangdong provinces; variegated laver is the main one in the north of the Yangtze River, mainly concentrated in Jiangsu and Shandong provinces. Among them, the cultivation area of ​​laver in Jiangsu Provinc...

Claims

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Application Information

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IPC IPC(8): C07K14/405C07K1/18C07K1/16C08B37/00
CPCC07K14/405C08B37/00
Inventor 王广策牛建峰冯建华何林文
Owner 南通中国科学院海洋研究所海洋科学与技术研究发展中心
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