Lactobacillus-fermented tea leaf extract for cigarettes and preparation method of lactobacillus-fermented tea leaf extract for cigarettes
A technology of lactic acid bacteria fermentation and lactic acid bacteria, applied in the preparation of tobacco, tobacco, application, etc.
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Embodiment 1
[0014] 1) Tea extract: take black tea leaves, add distilled water, heat and reflux for extraction for 2 hours according to the ratio of tea leaves to water to liquid: 1:8, then leave the extract to cool and then filter through a filter cloth to obtain tea extract;
[0015] 2) Lactic acid bacteria fermentation: use tea extract as a substrate, ferment it in an aseptic airtight state, the conditions of fermentation are: the volume of sucrose added is 6% of the volume of tea extract, and the inoculum volume of lactic acid bacteria is 6% of the volume of tea. 1.0% of the extract, the fermentation temperature is 28°C, and the fermentation time is 3 days;
[0016] 3) Post-treatment of the fermented product: After filtering to remove large particles of impurities, concentrate the filtrate for the first time at -0.01~-0.1Mpa at 80°C, add 4 times the volume of alcohol solution with a mass fraction of 95%, and dissolve it back at -20°C Cooling and sedimentation treatment for 18 hours; us...
Embodiment 2
[0018] 1) Tea extract: take green tea leaves, add distilled water, heat and reflux for extraction for 1 hour according to the ratio of tea to water to liquid: 1:6, then let the extract stand for cooling and then filter it with a filter cloth to obtain tea extract;
[0019] 2) Lactic acid bacteria fermentation: use tea extract as a substrate, ferment it in an aseptic airtight state, the conditions of fermentation are: the volume of sucrose added is 6% of the volume of tea extract, and the inoculum volume of lactic acid bacteria is 6% of the volume of tea. 4.0% of the extract, the fermentation temperature is 37°C, and the fermentation time is 2 days;
[0020] 3) Post-treatment of the fermented product: After filtering to remove large particles of impurities, concentrate the filtrate for the first time at -0.01 ~ -0.1Mpa at 80°C, add 3 times the volume of alcohol solution with a mass fraction of 95%, and redissolve at -20°C Cooling and sedimentation treatment for 20 hours; filter...
Embodiment 3
[0022] 1) Tea extract: take oolong tea leaves, add distilled water, heat and reflux for extraction for 3 hours according to the ratio of tea and water to liquid of 1:10, then leave the extract to cool and then filter through a filter cloth to obtain tea extract;
[0023] 2) Lactic acid bacteria fermentation: use tea extract as a substrate, ferment it in an aseptic airtight state, the conditions of fermentation are: the volume of sucrose added is 6% of the volume of tea extract, and the inoculum volume of lactic acid bacteria is 6% of the volume of tea. 2.0% of the extract, the fermentation temperature is 30°C, and the fermentation time is 1 day;
[0024] 3) Post-treatment of the fermented product: After filtering to remove large particles of impurities, concentrate the filtrate for the first time at -0.01 to -0.1Mpa at 80°C, add 5 times the volume of alcohol solution with a mass fraction of 95%, and redissolve at -20°C Cooling and sedimentation treatment for 24 hours; use filt...
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