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A kind of dna identification method of jelly jelly and Xihuang grass

A technology of Xihuangcao and Xihuangcao, which is applied in the field of identification of source varieties of Chinese medicinal materials, can solve problems such as the identification research of unseen medicinal materials jellygrass and its counterfeit products, the identification method of undiscovered medicinal materials jelly and Xihuangcao, etc.

Active Publication Date: 2018-01-19
GUANGZHOU WANGLAOJI MAJOR HEALTH IND +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there has been no research on the identification of the medicinal material jellygrass and its counterfeit products using this method, and so far no report on the identification method of the medicinal materials jellygrass and Xihuangcao

Method used

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  • A kind of dna identification method of jelly jelly and Xihuang grass
  • A kind of dna identification method of jelly jelly and Xihuang grass
  • A kind of dna identification method of jelly jelly and Xihuang grass

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Embodiment 1: Identification of the original plants of Jellygrass and Xihuangcao

[0100] 1. Method:

[0101] (1) Provide samples:

[0102] A total of 26 samples of original plants and medicinal materials of jelly jelly from different origins and 8 samples of original plants and medicinal materials of Xihuang grass were collected (see Table 1 for details).

[0103] Table 1. Sample information table

[0104]

[0105] a Indicates that the sample is the original plant, b Indicates that the sample is medicinal material.

[0106] (2) DNA sample extraction:

[0107] All samples were dried at low temperature (for example, 40°C), and about 30 mg of dried leaves were taken and ground with a high-throughput tissue grinder (Ningbo Xinzhi) at 50 Hz for 120 s, and plant genomic DNA kit (Tiangen Biochemical Technology (Beijing) Co., Ltd. , Cat#DP3112, Bioteke Corporation) to extract total DNA.

[0108] (3) PCR amplification:

[0109] PCR amplification primers are:

[0110...

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Abstract

The invention relates to a method for identifying DNA of Chinese mesona herb and rabdosia lophanthide. The method comprises the following steps: extracting DNA from a sample; by taking the DNA sample as a template, amplifying a segment with a TIS2 sequence, and performing sequencing analysis on the segment; and comparing sequences and analyzing by using a system development tree. The result shows that the sequences of Chinese mesona herb and rabdosia lophanthide have remarkable difference. By adopting the method, the mechanical material Chinese mesona herb and an adulterant rabdosia lophanthide can be accurately, efficiently and rapidly identified.

Description

technical field [0001] The disclosure belongs to the technical field of identification of source varieties of Chinese medicinal materials; in particular, the disclosure relates to a method of using the ITS2 sequence for the identification of Herba japonicus and Scutellaria japonicus. Background technique [0002] Herb jelly is the dry aboveground part of the Lamiaceae plant Mesona chinensis Benth. It is also known as fairy grass, fairy jelly, fairy grass, etc. Mainly produced in Guangdong, Guangxi, Zhejiang, Jiangxi and other provinces. "Materia Medica Qiuyuan" records that jelly jelly is astringent, sweet and cold in nature, good for summer heat, relieves heat and poison in Tibet, and cures alcoholism. Modern studies have shown that jelly jelly contains various chemical active ingredients such as jelly jelly polysaccharide, oleanolic acid, quercetin and flavonoids. It has the functions of anti-tumor, anti-inflammation, lowering blood sugar, protecting the liver, detoxifyi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895
Inventor 陈士林师玉华孙伟向丽方广宏翁少全李词周郑荣波黄晓丹杨光沈颖莉彭绍忠
Owner GUANGZHOU WANGLAOJI MAJOR HEALTH IND