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Preparation method of photonic crystal total reflection layer and rapid detection device for total number of bacteria based on the total reflection layer

A technology of photonic crystals and photonic crystal microspheres, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of increasing the cost of detection instruments, bulky grating, inconvenient to carry, etc., and achieve short detection time, high detection accuracy, and compact structure Effect

Active Publication Date: 2017-04-12
HARBIN INST OF TECH
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the grating is used to generate monochromatic light with a wavelength of 600nm due to the use of a grating in the rapid detection method of bacteria, but the grating is not only bulky and inconvenient to carry, but also expensive, which increases the cost of the detection instrument. Provide a method for preparing a photonic crystal total reflection layer and a rapid detection device for the total number of bacteria based on the total reflection layer

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  • Preparation method of photonic crystal total reflection layer and rapid detection device for total number of bacteria based on the total reflection layer

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specific Embodiment approach 1

[0012] Specific implementation mode 1: The method for preparing a photonic crystal total reflection layer described in this implementation mode includes the following steps:

[0013] Step 1, preparing photonic crystal microspheres with uniform monodispersity, the photonic crystal can generate a photonic band gap at 600nm;

[0014] Step 2. After centrifuging, washing and drying the photonic crystal microspheres, configure the photonic crystal microspheres into an ethanol aqueous suspension with a concentration of 3% to 6%. In the ethanol aqueous suspension, the volume of ethanol is Accounting for 15% to 25%;

[0015] Step 3, adding glucose to the aqueous ethanol suspension, and stirring evenly, the mass ratio of the added glucose to the aqueous ethanol suspension is 0.5:100 to 2.5:100;

[0016] Step 4. Cultivate the evenly stirred ethanol water suspension on a glass plate at a constant temperature of 65°C to 75°C for 3.5 to 4.25 hours. After the cultivation, take out the glass...

specific Embodiment approach 2

[0019] Embodiment 2: This embodiment is a further limitation on the preparation method of the photonic crystal total reflection layer described in Embodiment 1. In this embodiment, the photonic crystal described in step 1 is an opal structure or an inverse opal structure photonic crystal.

[0020] SiO with opal structure 2 Taking photonic crystal as an example, the diameter of the microsphere is 288nm. The diameter of photonic crystals with different structures has a specific relationship with the wavelength of the photonic band gap, which can be found in reference books.

specific Embodiment approach 3

[0021] Embodiment 3: This embodiment is a further limitation of the method for preparing the photonic crystal total reflection layer described in Embodiment 1. In the ethanol aqueous suspension described in Step 2, the concentration of photonic crystal microspheres is 4%.

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Abstract

The invention provides a preparation method of a photonic crystal total-reflection layer and a total-reflection layer-based fast detector for the total number of bacteria, relates to the bacteria number detection technology and aims at solving the problems that when a conventional rapid bacteria detection method is adopted, the large size and the high price can be caused due to the adoption of an optical grating for generating monochromatic light with the wavelength of 600nm. A photonic crystal microsphere is adopted to prepare an ethanol-water suspension, glucose is added in the ethanol-water suspension to obtain a mixture, the mixture is cultured on a glass plate at 70 DEG C, and the glass plate is taken out and encapsulated after 4 hours, and therefore, the photonic crystal total-reflection layer is obtained; the photonic crystal total-reflection layer is adopted to replace the optical grating to obtain the monochromatic light with the wavelength of 600nm, the monochromatic light is applied to the rapid bacteria number detection, the instrument stability is good, the size is small, the detector can be carried conveniently, and the cost is low. The fast detector is suitable for the bacteria number detection.

Description

technical field [0001] The invention relates to the technology for detecting the number of bacteria. Background technique [0002] Among the existing rapid detection methods for bacteria, one is the ATP (adenosine triphosphate) bioluminescence method. The main disadvantage of this method is that it cannot distinguish between microorganisms and non-microbial ATP, it cannot specifically distinguish the type of microorganisms, and the sample itself and ATP Certain ions contained in the extractant etc. will interfere with the determination of ATP and inhibit the luminescence. At the same time, this method requires a suitable ATP extraction agent, requires professional operation, and is greatly affected by free ATP and somatic cells, and there are many factors affecting the enzymatic reaction, resulting in insufficient accuracy of the detection results, and the reagents are expensive, which increases the detection efficiency. cost. Another rapid detection method for bacteria is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 李垚陈晓义赵九蓬张秋明徐洪波
Owner HARBIN INST OF TECH