Wide-waveband and large-field-depth microscope objective optical system used for flow cytometry

Abstract

The invention belongs to the field of design of imaging optical systems and discloses a wide-waveband and large-field-depth microscope objective optical system used for a flow cytometry. The wide-waveband and large-field-depth microscope objective optical system used for the flow cytometry aims at solving the problems that an existing microscope objective of the flow cytometry is narrow in waveband and small in field depth in the prior art. The wide-waveband and large-field-depth microscope objective optical system used for the flow cytometry comprises a first lens assembly, a second lens assembly, a cubic phase board, a third lens assembly, an eighth lens, a fourth lens assembly and a fifth lens assembly which are sequentially arranged along the optical axis from left to right; the fifth lens assembly is a plus lens assembly and comprises a binary diffraction optical element, the flow face, close to the flow cytometry, of the binary diffraction optical element is a binary optical diffraction face, the base face is a plane, the cubic phase board is located at the exit pupil position of the microscope objective optical system, and the face, deviating from a flow cytometry flow chamber, of the binary diffraction optical element is a cubic surface. After the binary diffraction optical element is introduced, chromatic aberration is well corrected. Meanwhile, after the cubic phase board is introduced, the field depth of the microscope objective is extended by twenty times to reach + / -16.8 micrometers.

Description

technical field [0001] The invention belongs to the field of imaging optical system design, in particular to a microscopic objective optical system for flow cytometry with wide band and large depth of field. Background technique [0002] As a new type of cell quantitative analysis and sorting technology, flow cytometry can rapidly analyze single cells or other biological particles at the subcellular level at the functional level. The microscope objective lens is an important part of the flow cytometer, which directly determines the observation effect of the cytometer. [0003] Flow cytometry uses the laser to excite stained cells to observe the cells. Most of the traditional cytometer microscope objectives are monochromatic waves or narrow bandwidths, which cannot fully meet the wide-band fluorescence imaging, resulting in the loss of cell information. . Moreover, due to the contradiction between the numerical aperture of the microscope objective lens and the depth of fiel...

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to propose a kind of optical system for the microscopic objective lens of flow cytometry wide band and large depth of field, solve the problem that the microscopic objective lens of flow cytometer has narrow wave band and small depth of field in the prior art

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