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Modified microorganisms and methods of making butadiene using same

A technology of butadiene and microorganisms, applied in the field of microorganisms, can solve problems such as expensive catalysts, toxicity, etc.

Inactive Publication Date: 2015-03-25
布拉斯科南美公司(巴西)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other butadiene manufacturing methods involve toxic and / or expensive catalysts, highly flammable and / or gaseous carbon sources, and high temperatures

Method used

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  • Modified microorganisms and methods of making butadiene using same
  • Modified microorganisms and methods of making butadiene using same
  • Modified microorganisms and methods of making butadiene using same

Examples

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example 1

[0239] Example 1 : Modified microorganisms for the production of butadiene.

[0240] Microorganisms such as bacteria can be genetically modified to produce butadiene from fermentable carbon sources including, for example, glucose.

[0241] In an exemplary method, a microorganism can be genetically engineered by any method known in the art to include: i.) one or more enzymes in the coding pathway that catalyze the conversion of a fermentable carbon source to acetyl-CoA and propionyl-CoA polynucleotides, and / or polynucleotides for enzymes in one or more coding pathways that catalyze the conversion of acetyl-CoA and propionyl-CoA to butadiene; ii.) in one or more coding pathways that catalyze the conversion of fermentable carbon sources to croton A polynucleotide for an enzyme that converts crotonyl-CoA, and / or a polynucleotide for an enzyme in one or more coding pathways that catalyzes the conversion of crotonyl-CoA to butadiene; or iii.) a polynucleotide that catalyzes a ferm...

example 2

[0243] Example 2 : Fermentation of carbon sources by genetically modified microorganisms to produce butadiene.

[0244] A genetically modified microorganism produced as in Example 1 above can be used to ferment a carbon source to produce butadiene.

[0245] In an exemplary method, a fermentable carbon source (e.g., 9 g / L glucose, 1 g / L KH 2 PO 4 , 2g / L (NH 4 ) 2 HPO 4 , 5mg / L FeSO 4 ·7H 2 O, 10mg / L MgSO 4 ·7H 2 O, 2.5mg / L MnSO 4 ·H 2 O, 10mg / L CaCl 2 ·6H 2 O, 10mg / L CoCl 2 ·6H 2 0, and 10 g / L yeast extract) pre-sterilized medium was fed into the bioreactor.

[0246] During fermentation, anaerobic conditions are maintained by, for example, sparging nitrogen into the medium. A temperature suitable for fermentation (eg, about 30°C) is maintained using any method known in the art. An approximate physiological pH (eg, about 6.5) is maintained by, for example, automatic addition of sodium hydroxide. The bioreactor is stirred eg at about 50 rpm. Let the fermentati...

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PUM

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Abstract

The present disclosure generally relates to microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene. Also provided are methods of using the microorganisms in industrial processes including, for use in the production of butadiene and products derived therefrom.

Description

Background technique [0001] Butadiene (1,3-butadiene, CH 2 =CH-CH=CH 2 , CAS 106-99-0) are linear, conjugated 4-carbon hydrocarbons typically produced (along with other 4-carbon molecules) by steam cracking of petroleum-based hydrocarbons. This process involves harsh conditions and high temperatures (at least about 850°C). Other butadiene manufacturing methods involve toxic and / or expensive catalysts, highly flammable and / or gaseous carbon sources, and high temperatures. Several million tons of butadiene-containing polymers are produced worldwide each year. Butadiene can be polymerized to form polybutadiene, or reacted with hydrogen cyanide (hydrogen cyanide) in the presence of a nickel catalyst to form adiponitrile (a precursor to nylon). More commonly, however, butadiene is polymerized with other olefins to form copolymers such as acrylonitrile-butadiene-styrene (ABS), acrylonitrile-butadiene (ABR) or styrene-butadiene (SBR ) copolymer. Contents of the invention [0...

Claims

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Application Information

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IPC IPC(8): C12P5/02C12N1/12C12N1/20
CPCC12P5/02C12N15/52C12N9/88C12Y402/01127Y02E50/30C12P5/026C12Y101/01C12Y101/01001C12Y102/01C12Y402/01
Inventor 马特乌斯·施莱纳·加尔赛斯罗佩斯阿芙拉姆·迈克尔·斯洛维奇尤里·埃斯特拉达·戈维亚约翰纳·林科内斯·佩雷斯卢卡斯·彭德森·帕里奇
Owner 布拉斯科南美公司(巴西)