Preparation method of chlorella pyrenoidosa polyphenol

A technology of chlorella pyrenoidosa and its operation method, which is applied in the field of preparation of chlorella pyrenoidosa polyphenols, and achieves the effects of safe process flow, convenient industrial production, and low production cost

Inactive Publication Date: 2015-04-01
HEILONGJIANG JOHNSUN BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the extraction process of algae polyphenols, there are still disadvantages such as the extraction steps are responsible, and the efficiency is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A preparation method of Chlorella pyrenoidosa polyphenols is characterized in that: the main process flow is: pulverization, mixing, extraction, and filtration, and the specific operation method sequentially follows the following steps:

[0020] (1) Crushing: Collect Chlorella pyrenoidosa, wash, dry, and crush through a 40-mesh sieve;

[0021] (2) Mixing: Weigh Chlorella pyrenoidosa, then add an ethanol solution with a concentration of 10-80%, with a material-to-liquid ratio of 20-100ml / g, stir evenly to prepare a mixed solution;

[0022] (3) Extraction: The above-mentioned mixed liquid is extracted in a microwave field with a microwave power of 500-1500W, and the microwave processing time is 20-100s to prepare an extraction filtrate;

[0023] (4) Filtration: freeze-drying the above-mentioned filtrate to obtain a polyphenol extract of Chlorella pyrenoidosa.

Embodiment 2

[0025] Extract seaweed polyphenols as follows:

[0026] (1) Collect Chlorella pyrenoidosa, wash, dry, and crush through a 40-mesh sieve;

[0027] (2) Weigh out Chlorella pyrenoidosa, then add a 30% ethanol solution with a material-to-liquid ratio of 10ml / g, and stir properly.

[0028] (3) The mixture of step 2 is extracted in a microwave field with a microwave power of 800W, and the microwave processing time is 20s.

[0029] (4) Filter, freeze-dry the filtrate to obtain polyphenol extract.

[0030] The extraction rate of seaweed polyphenols is 2.51%.

Embodiment 3

[0032] Extract seaweed polyphenols as follows:

[0033] (1) Collect Chlorella pyrenoidosa, wash, dry, and crush through a 40-mesh sieve;

[0034] (2) Weigh out Chlorella pyrenoidosa, and then add a 20% ethanol solution with a material-to-liquid ratio of 20ml / g, and stir properly.

[0035] (3) The mixture of step 2 is extracted in a microwave field with a microwave power of 800W, and the microwave processing time is 40s.

[0036] (4) Filter, freeze-dry the filtrate to obtain polyphenol extract.

[0037] The extraction rate of seaweed polyphenols was 3.08%.

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Abstract

The invention discloses a preparation method of chlorella pyrenoidosa polyphenol. The chlorella pyrenoidosa polyphenol is extracted by using the preparation method comprising the following steps of smashing, mixing, extracting and filtering. The preparation method has the advantages of high absorption rate, low cost, convenience for industrial production and the like.

Description

Technical field: [0001] The invention relates to the technical field of algae polyphenol extraction, in particular to a preparation method of chlorella pyrenoidosa polyphenols. Background technique [0002] Seaweed polyphenols is a general term for polyphenolic compounds extracted from seaweed, and has become a research hotspot in the field of phenolics because of its unique physiological functions. Polyphenols are a general term for a large class of compounds with different structures and containing multiple hydroxyl groups, which are widely found in land plants. However, with the application of modern analysis and separation techniques to the development of seaweed resources, seaweed polyphenols have increasingly attracted people's attention. According to the existing research, seaweed polyphenols are abundant in seaweeds such as Sargassum, Fucus, and Shuiyun. Polyphenols are extracted from algae. [0003] Seaweed polyphenols are a large class of compounds with differen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/02
CPCA61K36/05A61K2236/333A61K2236/51
Inventor 陈喜军
Owner HEILONGJIANG JOHNSUN BIOLOGICAL ENG CO LTD
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