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Detection method of related substances in vegetable drug extract-scutellarin

A technology for detection of scutellarin and its detection method, which is applied in the field of detection of related substances in the botanical extract scutellarin, and can solve the problems of no identification of substances and determination of content limits of related substances, etc.

Active Publication Date: 2015-04-01
YUNNAN BIOVALLEY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although breviscapine is now used as a traditional Chinese medicine raw material, its preparations have been used as a single compound, especially as injections and freeze-dried powder injections, such as breviscapine tablets, breviscapine injection, and breviscapine for injection. Therefore, there is no content limit determination of related substances, which is far from the clear identification and content limit determination requirements of related substances in foreign chemicals.

Method used

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  • Detection method of related substances in vegetable drug extract-scutellarin
  • Detection method of related substances in vegetable drug extract-scutellarin
  • Detection method of related substances in vegetable drug extract-scutellarin

Examples

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Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1. Separation and identification of four related substances:

[0060] The scutellarin APIs provided by 7 companies and the scutellarin tablets provided by 11 companies were used for high performance liquid chromatography detection, and the APIs with higher content of related substances were selected for the separation and identification of related substances. Take 20g of breviscapine API, dissolve it in DMF, and separate it by high performance liquid chromatography. The chromatographic column is Shimadzu Hedera ODS-P 30×250mm, 50ml / min, and the detection wavelength is 335nm. Using 26% methanol-0.05% formic acid aqueous solution as the eluent, four related substances were received according to UV light. Compounds 1, 2, 3, 4 were isolated. Compounds 1, 2, 3 and 4 were subjected to NMR spectra and high-resolution mass spectrometry, respectively. Compounds 1, 2, 3 and 4 were identified as: 6-hydroxykaempferol-7-O-β-glucuronic acid, scutellarin, scutellarin and ...

Embodiment 2

[0063] Embodiment 2. Separation and identification of four related substances:

[0064] The scutellarin APIs provided by 7 companies and the scutellarin tablets provided by 11 companies were used for high performance liquid chromatography detection, and the APIs with higher content of related substances were selected for the separation and identification of related substances. Take 20g of breviscapine API, dissolve it in DMF, and separate it by high performance liquid chromatography. The chromatographic column is Shimadzu Hedera ODS-P 30×250mm, 50ml / min, and the detection wavelength is 335nm. Using 12% acetonitrile-0.05% formic acid aqueous solution as the eluent, four related substances were received according to ultraviolet. Compounds 1, 2, 3, 4 were isolated. Compounds 1, 2, 3 and 4 were subjected to NMR spectra and high-resolution mass spectrometry, respectively. Compounds 1, 2, 3 and 4 were identified as: 6-hydroxykaempferol-7-O-β-glucuronic acid, scutellarin, scutellar...

Embodiment 3

[0067] Embodiment 3. Separation and identification of four related substances:

[0068] The scutellarin APIs provided by 7 companies and the scutellarin tablets provided by 11 companies were used for high performance liquid chromatography detection, and the APIs with higher content of related substances were selected for the separation and identification of related substances. Take 20g of breviscapine API, dissolve it in DMF, and separate it by high performance liquid chromatography. The chromatographic column is Shimadzu Hedera ODS-P 30×250mm, 50ml / min, and the detection wavelength is 335nm. Using 26% methanol-0.05% formic acid aqueous solution as the eluent, four related substances were received according to UV light. After purification by semi-preparative high performance liquid chromatography, compounds 1, 2, 3, and 4 were isolated. Compounds 1, 2, 3 and 4 were subjected to NMR spectra and high-resolution mass spectrometry, respectively. Compounds 1, 2, 3 and 4 were iden...

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Abstract

The invention provides related substances in a vegetable drug extract-scutellarin and a detection method of the related substances. According to the method, an accurate detection result can be obtained, the interference caused by other substances is avoided, a double effect is achieved, an identification result is relatively objective, accurate, precise and stable.

Description

technical field [0001] The invention provides a detection method for related substances in botanical extract scutellarin. Background technique [0002] Botanical medicines often contain a variety of chemical components, and these inherent chemical components are the material basis for herbal medicines to exert their medicinal effects. The method of detecting its complex components, the fingerprint came into being. [0003] The identification of "finger printing" comes from forensic science, and each person's fingerprint is different in the structure of small details. Based on these differences, by means of "matching", the characteristics that identify each individual can be determined. With the development of modern biotechnology, DNA fingerprinting has emerged. Through DNA fingerprint analysis, it can identify and identify human, animal, plant and other organisms. [0004] The use of fingerprints (especially chromatographic fingerprints) for quality control of botanical...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 伏继萍吴丽明杜江林艳和陈彬张雯洁张赟华董媛
Owner YUNNAN BIOVALLEY PHARMA CO LTD
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