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Use of dihydrochalcone glucoside as active ingredient for whitening and improving dull skin quality

A technology of dihydrochalcone glycosides and active ingredients, which is applied in the field of dihydrochalcone glycosides as active ingredients for whitening and improving dull skin quality, and can solve the problem of single ingredients

Active Publication Date: 2017-06-16
FOSHAN GOLDEN HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The dihydrochalcone glycoside compounds involved in the present invention have definite and single ingredients, among which naringin dihydrochalcone (C) is known to have exfoliating and acne-removing effects (refer to Patent Document 6), but currently there is no The dihydrochalcone glycoside compound involved in the present invention is used in the report of preventing or treating melanin, improving skin quality in food, cosmetics or pharmaceuticals

Method used

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  • Use of dihydrochalcone glucoside as active ingredient for whitening and improving dull skin quality
  • Use of dihydrochalcone glucoside as active ingredient for whitening and improving dull skin quality
  • Use of dihydrochalcone glucoside as active ingredient for whitening and improving dull skin quality

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Tyrosinase Inhibition Test

[0038] 1. Determination of tyrosinase activity

[0039] 15 μL 5% DMSO solution, 60 μL 0.25 mmol / L L-Dopa, 195 μL pH 6.8 phosphate buffer solution, shake well. After incubating at 37°C for 5 minutes, add 30 μL of tyrosinase solution and mix immediately. Measure the absorbance value at 475nm with a multi-functional microplate reader at a constant temperature of 37°C, make three parallel samples for each sample, and use the system without enzyme as a blank to draw a straight line of absorbance growth with time, and calculate it from the slope of the line Enzyme activity A 0 .

[0040] 2. Determination of tyrosinase enzyme activity under the action of samples

[0041] 15 μL samples of different concentrations (5% DMSO), 60 μL 0.25 mmol / L L-Dopa, 195 μL pH 6.8 phosphate buffer solution, shake well. After incubating at 37°C for 5 minutes, add 30 μL of tyrosinase solution and mix immediately. Measure the absorbance value at 475nm with a multi...

Embodiment 2

[0051] Inhibition of melanin production in B16 melanoma cells

[0052] Using NaOH cracking method. The day before the detection, the cells were subcultured to make their confluence 30%-50%, and DMEM-high glucose medium was used for detection. For example, a 6-well plate is used, the diameter of each well is 34.8mm, and the growth area is 9.5cm 2 . The amount of medium used is 2000 μL. Add samples for processing the next day, when the confluence reaches 80%-90%, the cell volume is 1×10 6 / hole. When the eyes see obvious color changes in the medium, and the microscope observes black precipitates in the cells. After about 48 hours, the cells were collected for detection. Harvest 1×10 6 Wash the cells twice with PBS, add 200 μL redistilled water to suspend the precipitate, add 1 mL ethanol:ether (1:1) mixture, let stand at room temperature for 20 min, centrifuge at 3000 r / min for 5 min, add 1 mol / L NaOH l to the precipitate mL (containing 10% DMSO), incubated in a water ba...

Embodiment 4

[0058] Preparation of gel-like peel-off mask

[0059] In 65g of pure water, add 5g of carboxymethylcellulose and 10g of polyvinyl alcohol, dissolve while heating, add 0.1g of hesperidin dihydrochalcone and 19g of ethanol at the same time, add a small amount of citrus essence, Makes into a gel-like peel-off mask.

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PUM

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Abstract

The invention discloses applications of dihydrochalcone and a dihydrochalcone glucoside compound as active ingredients used for whitening skin and improving dark and gloomy skin. Structural formula of the dihydrochalcone glucoside compound is represented by formula (I), wherein R1, and R2 are used for representing H, OH, or methoxy respectively, R3 is used for representing H, glucosyl, rutinose group, or neohesperidose group. The dihydrochalcone glucoside compound can be used as food, cosmetics, or pharmaceuticals used for preventing or treating melanin pigmentation, and improving skin; and is excellent in effects.

Description

technical field [0001] The present invention relates to using dihydrochalcone glycoside compounds as active ingredients for whitening and improving dull skin quality, and specifically relates to said dihydrochalcone glycoside compounds as tyrosinase inhibitors, which can effectively hinder melanin production and promote The reduction of pigmentation, and its application in the preparation of food, cosmetics or pharmaceuticals for preventing or treating melanin and improving skin quality. Background technique [0002] In today's society, the pace of life and work continues to accelerate, environmental pollution and the destruction of the ozone layer cause the formation and metabolism of melanin to become chaotic, resulting in an increasing number of people suffering from various black spots; activated oxygen produced and ingested from inside and outside the body is oxidized Problems such as skin aging caused by reactions and skin coloration caused by ultraviolet rays are also...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/60A61K8/35A61Q19/02A61K31/7034A61K31/12A61P17/00A23L33/10
Inventor 周金林卢宇靖黄宝华
Owner FOSHAN GOLDEN HEALTH TECH CO LTD
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