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Directional cultivation of adventitious roots of ginseng by dynamic balanced nutrition

A dynamic balance and nutrition technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of ginseng adventitious roots, nutritional imbalance, etc.

Active Publication Date: 2017-01-18
珲春华瑞参业生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to solve the problem of unbalanced nutrition at each stage of cultivating ginseng adventitious roots in bioreactors, improve the yield of ginseng adventitious roots and the content of secondary metabolites, and provide a method for directional cultivation of ginseng adventitious roots by dynamic balanced nutrition

Method used

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  • Directional cultivation of adventitious roots of ginseng by dynamic balanced nutrition
  • Directional cultivation of adventitious roots of ginseng by dynamic balanced nutrition
  • Directional cultivation of adventitious roots of ginseng by dynamic balanced nutrition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 The acquisition of ginseng adventitious root

[0035] Select 6-year-old garden ginseng from Changbai Mountain (Ji'an County, Tonghua City, Jilin Province), cut into pieces, and use MS medium to induce callus, and use 3\4 SH medium + IBA5.0mg / L + sucrose 30g / L, induced adventitious roots, subcultured.

Embodiment 2

[0036] The preparation of embodiment 2 initial culture medium and nutrient supplement solution

[0037] 1. Preparation of culture medium

[0038] 1) Initial medium

[0039] 3 / 4SH medium + potassium indolebutyrate (K-IBA ) 3mg / L + sucrose 30g / L

[0040] 2) Nutrient additive solution

[0041] Nutrient additive solution (1):

[0042] In every liter of 3 / 4SH medium, add naphthaleneacetic acid (C 12 h 10 o 2 ) 2.7mg / L, methyl jasmonate (C 13 h 20 o 3 ) 240mg / L, salicylic acid 240 mg / L, sucrose (C 12 h 22 o 11 ) 90g×3 / L.

[0043] Nutrient additive solution (2):

[0044]In every liter of 3 / 4SH medium, add naphthaleneacetic acid (C 12 h 10 o 2 ) 2.7mg / L, methyl jasmonate (C 13 h 20 o 3 ) 204mg / L, salicylic acid 240mg / L, sucrose (C 12 h 22 o 11 ) 120g / L.

Embodiment 3

[0045] Example 3 Bioreactor Dynamic Balanced Nutrition Method Oriented Cultivation of Ginseng Adventitious Roots

[0046] 1) Add 12L of the initial culture medium into the 18L bubbling bioreactor, add 6L of the nutrient supplement into the liquid replenisher, and sterilize the bioreactor and the liquid replenisher at a temperature of 120°C and a pressure of 0.08Mpa1;

[0047] 2) The adventitious roots were cut into 1cm pieces and inoculated into the bioreactor;

[0048] 3) Ventilate and incubate at 23±2°C;

[0049] 4) After culturing until the 10th day, add 6L of nutrient supplement solution (1) dropwise, and finish dropping in 3-5 days;

[0050] 5) Continue culturing for 42 days to harvest adventitious roots.

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Abstract

The invention discloses a method for orientationally cultivating ginseng adventitious roots by dynamic balance alimentation. The method comprises the following steps: (1) setting the volume ratio of an primary culture medium to a nutrition additive solution to be (2-3): 1, wherein the primary culture medium is a 3 / 4SH culture medium+ IBA 3mg / L+ saccharose 20-35g / L, and the nutrition additive solution is a 3 / 4SH culture medium+ saccharose 90-120g / L; (2) adding the primary culture medium into a bubbling type bioreaction tank, and performing inoculation; (3) feeding air for cultivation; (4) after cultivation is performed after 5-10 days, dropping the nutrition additive solution, and finishing dropping after 3-5 days; (5) continuously cultivating for 30-42 days, and obtaining the adventitious roots. Nutritional components can be dropped in real time according to requirements; the bubbling type bioreaction tank realizes pneumatic stirring under the action of rising bubbles, so that the nutrition additive solution can be fully mixed into a basic culture medium; the adventitious roots can be orientationally obtained, the cultivation time is shortened, and the yield of the adventitious roots and the content of total saponins are increased or the content of target saponins monomers in a component is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for cultivating adventitious roots of ginseng in a bioreactor. Background technique [0002] Ginseng ( Panax ginseng C. A. Meyer) is a plant of the genus Panax L. of the family Araliacede. It is one of the famous "Three Treasures of Northeast China", and it is also a traditional Chinese herbal medicine in my country. It is well-known both at home and abroad. Li Shizhen, a great physician and pharmacist in the Ming Dynasty in my country, gave a detailed introduction to the efficacy of ginseng in his epic masterpiece "Compendium of Materia Medica", which established the reputation of ginseng as the "king of all herbs". Ginseng mostly grows in hillsides of coniferous forests or mixed forests with a small temperature difference between day and night and an altitude of 500-1100 meters. In my country, it is mainly distributed in the three northeastern provinces and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 金秀莲柳青桂英丛凡华
Owner 珲春华瑞参业生物工程股份有限公司
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