Upper-jiao fluid retention stagnating in the interior rat model and construction method thereof

Abstract

The invention discloses an upper-jiao fluid retention stagnating in the interior rat model construction method. The method includes the steps of (1) placing an SPF Wistar rat in a purification cage with separate air supply and air exhaust, feeding the rat with common food and enabling the rat to eat food and drink water freely; (2) subcutaneously injecting the Wistar rat of step (1) with isoproterenol of 20 mg / kg on the first day, 10 mg / kg on the second day, 5 mg / kg on the third day and 3 mg / kg / d from the fourth day for 20 days and observing for two weeks; (3) arranging a trachea built-in catheter in the trachea of the Wistar rat; (4) placing the Wistar rat with the trachea built-in catheter in a freezer at the temperature of 0 DEG C for 4 hours every day to accept cold stimulation for seven consecutive days to obtain a upper-jiao fluid retention stagnating in the interior rat model. The upper-jiao fluid retaining rat model construction method solves the problem of no upper-jiao fluid retention stagnating in the interior rat model currently, provides a new method for normal, standard and scientific evaluation of traditional Chinese medicine and has the advantages of being simple in model construction, easy to operate and popularize, similar to clinic and the like.

Description

technical field [0001] The invention relates to a Shangjiao Shuiyin internal stop rat model and a construction method thereof, belonging to the technical field of medical evaluation and detection. Background technique [0002] Traditional Chinese medicine believes that the lungs are located in the upper burner, so it is called "Hua Gai" and "the source of the water". The main physiological functions of the lungs are to control qi, control respiration, control dispersal and discharge, and regulate water passages. The running compress of body fluid is related to the venting and descending movement of the lungs. "Spleen Qi looses essence and returns to the lungs." The lungs distribute the body fluid to the orifices of the head and face, fur and interstitial tissues through the function of spreading. And by virtue of its subduing effect, the body fluid is transported to other viscera and poured into the bladder. In this way, the water and essence are distributed in all directi...

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Problems solved by technology

[0005] At present, there is no rat model of internal withdrawal of Shangjiao Shuiyin

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