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Method for screening or identifying herbicide-resistant rice via agarose matrix

A herbicide-resistant and agarose-resistant technology, which is applied in the field of crop cultivation, can solve the problems of unapplied screening and identification of unknown genes, many technical links, time-consuming and labor-intensive problems, etc., and is suitable for large-scale screening or identification, with low requirements for experimental conditions. The effect of perfect technical system

Inactive Publication Date: 2015-04-29
天津市农作物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is time-consuming and labor-intensive, and the concentration and uniformity of spraying are not easy to control, which affects the accuracy of the results.
However, the method of using molecular markers is costly, and there are many technical links, and there are certain requirements for equipment; it is effective for known genes, but cannot be applied to the screening and identification of unknown genes.

Method used

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  • Method for screening or identifying herbicide-resistant rice via agarose matrix
  • Method for screening or identifying herbicide-resistant rice via agarose matrix
  • Method for screening or identifying herbicide-resistant rice via agarose matrix

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Using agarose matrix to identify methods for cultivating herbicide-resistant rice:

[0035] (1) The rice seeds to be tested were obtained

[0036] Imazethapyr-sensitive rice J1 and imazethapyr-insensitive rice Jindao 263 were sowed in the field, Jindao 263 was detasseled at the full flowering stage, and the pollen of J1 was used for hybridization to harvest F1 generation seeds. The F1 generation seeds are planted, and the F2 generation seeds are mixed; the F2 generation plants are planted on a single plant, and the F3 generation seeds are harvested from a single plant.

[0037] (2) Seed soaking and germination

[0038] Put the above 100 seeds harvested from the F3 generation in sulfuric acid paper bags, mark different individual plants, soak them in warm water at 32°C, and place them together with the container in a light incubator at 32°C to keep warm, morning, noon and evening every day. Change the water three times, and sow after 3 days of dew.

[0039] (3) Medium...

Embodiment 2

[0049] The method for screening and cultivating herbicide-resistant rice using agarose matrix:

[0050] (1) The rice seeds to be tested were obtained

[0051] Soak 1kg of Jinjaponica 28 seeds in distilled water for 5 hours, change to 1% EMS solution and soak overnight (about 12 hours), rinse with water for 4 hours, then soak in water at 32°C for overnight cultivation, and sow them in the seedling field on the second day. After growing 4-5 leaves, transplant them to the field, and obtain M1 generation seeds after maturity. Sow all M1 generation seeds, transplant single plants, and harvest M2 generation seeds on a single plant (multiple plants can be harvested in order to reduce workload).

[0052] (2) Seed soaking and germination

[0053] Put the above-mentioned 100 single-plant (or multi-plant mixed) M2 generation seeds in sulfuric acid paper bags, mark them, soak them in warm water at about 32°C, and place them together with the container in a light incubator at 32°C to kee...

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Abstract

The invention discloses a method for screening or identifying herbicide-resistant rice via agarose matrix. The method for screening or identifying herbicide-resistant rice via agarose matrix comprises the following steps: soaking the seed and sprouting, preparing the culture medium, sowing, observing and counting. The method is as follows: pouring 2L of the water into the electromagnetism pot, adding 10g of agarose, stirring evenly, boiling until that the agar dissolves totally, adding the herbicide imazethapyr mother liquor, the final concentration is 0.1ml / L, shaking evenly and pouring the mixture into the flat culture dish, using after that the culture medium solidifies; marking the name of variety on the culture dish, selecting the seed with stared area for 50-55 pieces, evenly sowing the seeds on the culture medium, sowing the resistant and anti-resistant variety for two pieces for having positive and negative contrast, scribing, separating and marking; observing and counting after 3-4 days. The method can screen or identify the herbicide-resistant imazethapyr rice quickly, effectively and exactly. Very good technical means is offered for the herbicide-resistant imazethapyr rice breeding and purity identification.

Description

technical field [0001] The invention belongs to the field of crop cultivation and relates to a method for screening or identifying herbicide-resistant rice by using an agarose matrix. Background technique [0002] Rice is one of the main food crops in my country and even in the world, with an annual planting area of ​​about 30 million hectares and an output of 200 million tons. In the production process of rice, weeds compete with rice for resources, which not only reduces the yield of rice, but also affects the quality of rice. In order to control the growth of weeds, a large amount of manpower and material resources need to be invested every year. With the acceleration of urbanization and the transfer of a large number of rural labor forces, traditional production methods will gradually disappear, and rice production will face the problem of labor shortage. Therefore, the development of new cultivation models with high efficiency and low consumption, such as direct seedi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01C1/00A01G1/00A01H1/02A01H1/06
CPCA01G7/00A01C1/00A01G31/00
Inventor 吴健王晓静李军玲段骅苏京平姚微姚丙晨孙玥孙林静马忠友闫双勇王胜军刘学军
Owner 天津市农作物研究所
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