CDKN2A epitope peptide for detecting cervical cancer markers and application of CDKN2A epitope peptide

An antigenic epitope, cervical cancer technology, applied in animal/human peptides, tumor-specific antigens, tumor rejection antigen precursors, etc., can solve problems such as low sensitivity and poor specificity

Active Publication Date: 2015-04-29
刘林林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently reported autoantibody detection methods have low sensitivity and poor specificity, and the false negative rate can be as high as 50%.

Method used

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  • CDKN2A epitope peptide for detecting cervical cancer markers and application of CDKN2A epitope peptide
  • CDKN2A epitope peptide for detecting cervical cancer markers and application of CDKN2A epitope peptide
  • CDKN2A epitope peptide for detecting cervical cancer markers and application of CDKN2A epitope peptide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, kit preparation

[0030] 1. Reagents: See Tables 1-6 for reagent preparation.

[0031] Table 1 antigen coating buffer (400ml volume)

[0032]

[0033] Table 2 Wash buffer (400ml volume)

[0034]

[0035] Table 3 Stop buffer (100ml volume)

[0036]

[0037] Table 4 working antigen

[0038]

[0039] Table 5 Secondary Antibody Standard Solution

[0040]

[0041] Table 6 Substrate Chromogenic Solution

[0042]

[0043] 2. Operation

[0044] (1) Coating: The microplate should be washed 3 times with washing buffer, the working antigen should be diluted to the working concentration with the coating solution, and coated on the microplate, overnight at 4°C.

[0045](2) Add glutamic acid: wash 3 times with washing buffer, dilute glutamic acid with coating solution to a concentration of 100 μg / ml, 200 μl per well, incubate at 37°C or room temperature for 1 hour;

[0046] (3) Add plasma and quality control control (primary antibody): wash the ...

Embodiment 2

[0050] Example 2, CDKN2A Auto IgG Antibody Detection of Cervical Patients

[0051] 1. Sample collection: 203 cases of malignant cervical tumors confirmed by radiological examination and histological examination were selected from the Second Hospital of Jilin University and Provincial Cancer Hospital. All serum samples had not undergone any anti-cancer treatment before collection, and had comprehensive clinical data and information. At the same time, 154 healthy control samples were recruited. Clinical interviews and imaging examinations ruled out the possibility of other diseases of the cervix. The healthy group and the cervical cancer group are comparable in gender and age (P>0.05)

[0052] 2. Test results: CDKN2A autoantibody expression level (see Table 7): There is a statistical difference between the cervical cancer group and the healthy control group (t=-4.480, P<0.001).

[0053] ROC curve analysis: the area under the curve of the cervical cancer group was 0.637 (SE=0....

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Abstract

The invention relates to a CDKN2A epitope peptide for detecting cervical cancer markers and an application of the CDKN2A epitope peptide, and belongs to the technical field of biology. An amino acid sequence of the CDKN2A epitope peptide for detecting the cervical cancer markers is as shown in SEQ ID NO:1; and the CDKN2A epitope peptide for detecting the cervical cancer markers is applied to preparation of an early diagnosis kit for cervical cancer. The CDKN2A epitope peptide has the advantages that the autoantibody expression level in serum and plasma of a patient with the cervical cancer is detected and a corresponding reagent is developed; the dangerousness of the cervical cancer is predicted; and a foundation is laid for preparation of the early diagnosis kit for the cervical cancer.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a CDKN2A polypeptide fragment with detection cervical cancer markers screened by bioinformatics simulation. Population epidemiological detection has verified its detection sensitivity and specificity, and is further used for the preparation of cervical cancer Early diagnostic kits lay the groundwork. Background technique [0002] A large number of studies have shown that tumor-associated antigens in serum or plasma can induce the body to produce autoantibodies, and there are both tumor antigens and autoantibodies against the tumor antigens in the serum of cancer patients. Therefore, both antibodies can be used to detect tumor antigens, and antigens can also be used to detect tumor antigen autoantibodies, but the specificity and sensitivity of using tumor autoantibodies to detect tumors are much higher than using tumor antigens to detect tumors. Many tumor-associated antig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47G01N33/68G01N33/574G01N33/564
CPCC07K14/4748G01N33/57411G01N33/689
Inventor 刘林林
Owner 刘林林
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