Cell line resistant to A subgroup avian leukosis virus as well as construction method and application thereof

Abstract

The invention discloses a cell line resistant to an A subgroup avian leukosis virus as well as a construction method and application thereof, and belongs to the technical field of agricultural biology. The cell line resistant to the A subgroup avian leukosis virus is named after an anti ALV-A chicken embryo fibroblast cell line DF-1 / A, and is conserved in Wuhan University, China of China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: C2014180 from October 15th, 2014; the cell line resistant to the A subgroup avian leukosis virus is applied to the diagnosis of the A subgroup avian leukosis virus. The cell line resistant to the A subgroup avian leukosis virus disclosed by the invention can achieve stable passage and be stored for a long term, is helpful for accurately identifying subgroup classification of ALV by using the characteristic of ALV superinfection, and is applied to the diagnosis of the A subgroup avian leukosis virus.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a cell line against subgroup A avian leukosis virus and its construction method and application. Background technique [0002] At present, avian leukemia is seriously harmful to my country's poultry industry, and various pathological phenotypes such as lymphoma, myeloid cell tumor type and hemangioma type have appeared. and other neoplastic diseases. The disease is mainly transmitted vertically. Effective measures to control the disease are to reduce the infection rate of breeder flocks and establish leukemia-free breeder flocks. Eliminate virus-positive chickens to purify and eliminate ALV in chicken flocks. Among the exogenous ALVs, the J subgroup, A subgroup and B subgroup avian leukosis virus have obvious pathogenicity to chickens, and are also very harmful to poultry production. [0003] At present, domestic and foreign methods for the detection and id...

AI Technical Summary

Problems solved by technology

[0003] At present, domestic and foreign detection and identification methods for the pathogen of avian leukemia mainly include cell culture, ELISA, indirect immunofluorescence test, PCR, etc. Among them, ELISA is the most widely used clinically, and its advantage is that the method is relatively simple to operate and commercialized. Detection kits are available, but the disadvantage is that endogenous and exogenous ALV cannot be distinguished; indirect immunofluorescence test is relatively intuitive, but often requires the use of specific antibodies (mAbs)

Although the ADOL laboratory in the United States has some specific chicken strains, the primary cells produced by them can limit the growth of specific exogenous avian leukosis virus respectively, but the rarity of these strains of chickens limits their effectiveness.

Currently, there are no reports of cell lines that can specifically restrict subgroup A avian leukosis virus

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