Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of plant endosperm-specific promoter and its application

A specific promoter and promoter technology, applied in the field of plant promoters, can solve the problems of material and energy consumption, original metabolic imbalance, gene silencing, etc.

Active Publication Date: 2018-07-27
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of constitutive promoters to promote the expression of the target gene has played a certain role in promoting the study of gene function, but because the target gene will be continuously expressed in all tissues, it not only consumes a large amount of material and energy in the cell, but also accumulates excess products. It will also lead to the imbalance of the original metabolism, hinder the normal growth and development of plants, and even lead to death
In addition, repeated use of constitutive promoters to drive expression of multiple foreign genes may result in gene silencing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of plant endosperm-specific promoter and its application
  • A kind of plant endosperm-specific promoter and its application
  • A kind of plant endosperm-specific promoter and its application

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0011] Embodiment 1: In this embodiment, the plant endosperm-specific promoter is the ZOU gene promoter, which is derived from the japonica subspecies rice Nipponbare. The size of the promoter is 2648bp, and its DNA sequence is shown in SEQ ID NO:1.

specific Embodiment approach 2

[0012] Specific embodiment two: the application of a plant endosperm-specific promoter in promoting the expression of a target gene in a plant according to this embodiment; the plant is rice.

Embodiment 1

[0013] Example 1. Method for obtaining plant endosperm-specific promoter and construction of pCXGUS-Pzou expression vector:

[0014] Genomic DNA was extracted from the leaves of rice variety Nipponbare, and the genomic DNA was used as a template to perform PCR amplification using primers AW32101 (with BamHI restriction site added) and AW32102 (with PstⅠ restriction site added), and the reaction system was as follows: 50 μL, consisting of the following components:

[0015]

[0016] The CR amplification conditions were: denaturation at 94°C for 5 minutes; followed by 30 cycles of 94°C, 2min, 55°C, 30sec, 72°C, 2min; 72°C extension reaction for 10min.

[0017] The rice variety Nipponbare was purchased from the China Rice Research Institute.

[0018] The product obtained by PCR amplification was sequenced, and the sequencing result showed that the plant endosperm-specific promoter had the nucleotide sequence of SEQ ID NO: 1 in the sequence table, which was the ZOU gene promote...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A plant endosperm-specific promoter and its application relate to a plant promoter and its application. The object of the present invention is to provide a promoter specifically expressed in rice endosperm tissue, an expression vector thereof, and uses of the promoter and the expression vector. The DNA sequence of the promoter is shown in SEQ ID NO:1. The invention transforms rice through an agrobacterium-mediated method, and successfully obtains transgenic plants. After PCR verification and hygromycin screening, it was shown that the rice endosperm-specific promoter had been integrated into the rice genome; GUS histochemical staining and GFP excitation light identification confirmed that the promoter of the ZOU gene was an endosperm-specific expression promoter. The application of the promoter in promoting the expression of the target gene in plants.

Description

technical field [0001] The present invention relates to a plant promoter and its application. Background technique [0002] A promoter is a specific nucleic acid sequence located upstream of a structural gene, which can regulate the initiation time and intensity of downstream structural gene transcription. According to the mode and function of transcription initiation in plants, promoters can be divided into three categories: constitutive promoters, tissue or organ-specific promoters, and inducible promoters. Constitutive promoters can promote the expression of genes in all tissues, which are continuous, do not show time-space specificity, and the expression levels of RNA and protein are relatively constant; gene transcription under the regulation of tissue or organ-specific promoters generally only occurs in In specific organs or tissues; inducible promoters need to be induced by certain physical or chemical signals to initiate the expression of the target gene. [0003] ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/82A01H5/00A01H6/46
Inventor 杨素欣豆明珠张耀华冯献忠
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S