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A molecular marker for identifying wheat grain peroxidase activity and its application

A technology of peroxidase and wheat grain, applied in the biological field, can solve the problems of the influence of genetic factors, the main effect QTL of wheat grain POD activity is not clear, and achieve the effect of accelerating the pace of breeding

Active Publication Date: 2017-03-15
XINJIANG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] POD activity is mainly affected by genotype, and the main QTL for POD activity in wheat grains is still unclear
Although environmental factors have some influence on POD activity, it is mainly affected by genetic factors

Method used

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  • A molecular marker for identifying wheat grain peroxidase activity and its application
  • A molecular marker for identifying wheat grain peroxidase activity and its application
  • A molecular marker for identifying wheat grain peroxidase activity and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1, the method for identifying wheat grain peroxidase (Peroxidase, POD) activity

[0051] 1. Molecular markers for identification of wheat grain peroxidase activity

[0052] 1. There are two allelic variant forms of the TaPod-D1 gene in wheat: one is shown in the 35th-1180th nucleotide molecule from the 5' end in sequence 5 of the sequence listing (named allele TaPod-D1a ); the other is shown in the 35th-1180th nucleotide molecule from the 5' end in sequence 6 of the sequence listing (named TaPod-D1b).

[0053] 2. Design specific primer pair 1 and primer pair 2 based on the two allelic variant forms of the TaPod-D1 gene as follows:

[0054] The upstream primer of primer pair 1 (sequence 1 of the sequence listing): 5'-ACGGGAGACGACGAGAAGCAAAG-3';

[0055] The downstream primer of primer pair 1 (sequence 2 of the sequence listing): 5'-TCGTGGAAGTGTAGGCGAAGA-3';

[0056] The upstream primer of primer pair 2 (sequence 3 of the sequence listing): 5'-GTGGCGCAGGGCCTGT...

Embodiment 2

[0068] Embodiment 2, verification of molecular markers

[0069] Experimental materials: 125 Chinese winter wheat main varieties (as shown in Table 1), the public can obtain from the National Germplasm Bank of the Institute of Crop Science, Chinese Academy of Agricultural Sciences.

[0070] The method for identifying wheat grain peroxidase activity described in Example 1 was used to verify 125 Chinese winter wheat main varieties. At the same time, various wheat varieties were planted in Anyang, Henan, and the POD activity of the wheat grains was tested after harvesting. The POD activity of each wheat material was tested twice. If the error of the two test results exceeds 10%, a third repeated test is required. The results are averaged.

[0071] The method for measuring the POD activity of the grain is as follows:

[0072] 1. Extraction of common wheat grain POD

[0073] (1) Select 1.2g wheat seeds, grind them with a high-throughput tissue grinder (Spex 2010Geno / Grinder, US; ...

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Abstract

The invention discloses a molecular marker used for identifying POD (peroxidase) activity of a wheat grain and an application of the molecular marker. The molecular marker comprises primer pairs shown in (1) and (2) as follows: (1) represents a primer pair 1 comprising single-chain DNA shown in SEQ ID No.1 and single-chain DNA shown in SEQ ID No.2; (2) represents a primer pair 2 comprising single-chain DNA shown in a SEQ ID No.3 and single-chain DNA shown in SEQ ID NO.4. As is proven by an experiment, the molecular marker can be used for quickly identifying TaPod-D1a and TaPod-D1b allelic variations and correlation between TaPod-D1a and TaPod-D1b allelic variations and POD activity of wheat, a wheat variety with relatively high POD activity is screened out, the cultivation speed of the new high-quality wheat variety is increased, and the molecular marker has great theoretical significance and economical value in assisting in selection of the wheat variety with high POD activity.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker for identifying wheat grain peroxidase activity and an application thereof. Background technique [0002] The color of flour and its products is an important content of genetic improvement of wheat quality, and color has become an important factor restricting the quality of flour and its products in my country. Peroxidase (Peroxidase, POD) activity is the main factor affecting the color and processing quality of flour products, which can cause color loss and whitening of flour and flour products during the processing and storage of wheat products. POD mainly releases molecular oxygen by catalyzing compounds containing carboxyl and ketone groups and unsaturated double bonds such as phenols and aromatics. The released molecular oxygen can oxidize the conjugated double bonds of pigment molecules in flour and noodle products into single bonds. , thus bleac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11A01G1/00
CPCA01G22/00C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 耿洪伟夏先春何中虎魏景欣
Owner XINJIANG AGRI UNIV
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