Preparation method of folic acid receptor-targeted ultrasound-enhanced nano-microbubbles
A technology of contrast-enhanced ultrasound and folic acid receptors, applied in the field of medical drugs, to achieve good biocompatibility, no in vitro toxicity, and good stability
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Embodiment 1
[0046] Example 1, preparation of folate receptor-mediated targeted ultrasound contrast-enhanced nano-microbubble core template nanoemulsion. Weigh 0.1125g glycerol (2.25% (w / v)) as an isotonic agent and co-emulsifier, and emulsifier 0.12g refined egg yolk lecithin (2.4%, w / v) and 0.03g poloxamer 188 ( 0.6%, w / v) and stabilizer 0.01g oleic acid (0.2%, w / v) were vortex mixed in 4ml ultrapure water to form a suspension, and the prepared suspension was placed at 37°C and incubated for 1h to prepare Form an emulsion, then add 100 μl perfluorooctyl bromide drop by drop under vortex mixing, dilute to 5ml with ultrapure water, put it into a 15ml Corning centrifuge tube, and quickly use the machine for ultrasound, the temperature is controlled at 20-30°C, and the temperature is controlled at 20-30°C, % Ultrasound intensity for 10 minutes, start the ultrasound for 10 seconds, and pause for 10 seconds to obtain a liquid fluorocarbon core template nanoemulsion. After the obtained liquid f...
Embodiment 2
[0049] Example 2, (1) Prepare folate receptor-mediated targeting ultrasound contrast-enhanced nano-microbubble core template nanoemulsion. Weigh 0.1125g glycerol (2.25% (w / v)) as an isotonic agent and co-emulsifier, and emulsifier 0.12g refined egg yolk lecithin (2.4%, w / v) and 0.04g poloxamer 188 ( 0.8%, w / v) and stabilizer 0.0125g oleic acid (0.25%, w / v) were vortex mixed in 4ml ultrapure water to form a suspension, and the prepared suspension was placed at 37°C and incubated for 1h to prepare Form an emulsion, then add 100 μl perfluorooctylbromide dropwise under vortex mixing, dilute to 5ml with ultrapure water, put it into a 15ml Corning centrifuge tube, and quickly put it on the machine for ultrasound, the temperature is controlled at 20-30 % Ultrasound intensity for 10 minutes, start the ultrasound for 10 seconds, and pause for 10 seconds to obtain a liquid fluorocarbon core template nanoemulsion. After the obtained liquid fluorocarbon core template nanoemulsion is dilut...
Embodiment 3
[0052]In Example 3, a folate receptor-mediated targeted ultrasound contrast-enhanced nano-microbubble core template nanoemulsion was prepared. Weigh 0.125g glycerol (2.5% (w / v)) as an isotonic agent and co-emulsifier, and emulsifier 0.11g refined egg yolk lecithin (2.2%, w / v) and 0.04g poloxamer 188 ( 0.8%, w / v) and stabilizer 0.0075g oleic acid (0.15%, w / v) were vortex mixed in 4ml ultrapure water to form a suspension, and the prepared suspension was placed at 37°C and incubated for 1h to prepare Form an emulsion, then add 125 μl perfluorooctyl bromide drop by drop under vortex mixing, dilute to 5ml with ultrapure water, put it into a 15ml Corning centrifuge tube, and quickly put it on the machine for ultrasound, the temperature is controlled at 20-30 % ultrasonic intensity for 10 minutes, ultrasonic start 15s, pause 15s, the liquid fluorocarbon core template nanoemulsion was prepared, the obtained liquid fluorocarbon core template nanoemulsion was diluted 10 times with 1ml o...
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