A method for screening, purifying and large-scale cultivation of Daphnia warmi

A technology of warm sword Daphnia and cultivation method, applied in the direction of climate change adaptation, fish farming, application, etc., can solve the problems of staying in natural reproduction, etc., and achieve the effects of moderate size, fast reproduction, and improved survival rate

Active Publication Date: 2017-01-18
NINGBO UNIV
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Problems solved by technology

But so far, there are almost no major species of copepods that can be cultured productively in large quantities. ) can be cultivated at low density, and the cultivation of these copepods mostly stays in the state of natural reproduction, and the large-scale artificial reproduction of copepods has not really been realized

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  • A method for screening, purifying and large-scale cultivation of Daphnia warmi
  • A method for screening, purifying and large-scale cultivation of Daphnia warmi
  • A method for screening, purifying and large-scale cultivation of Daphnia warmi

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specific Embodiment

[0024] A method for screening, purifying and large-scale cultivation of Daphnia warmis, which captures copepod populations from estuaries or breeding ponds at the junction of salty and fresh water; under set conditions, conducts directional screening to obtain Daphnia warmis; optimizes breeding of Daphnia warmi Under the conditions of large-scale continuous cultivation, the specific steps are as follows:

[0025] (1) Germplasm acquisition: In estuaries or breeding ponds at the junction of salty and fresh water, when the water temperature is 18-22°C (generally at the transition from spring to summer or late autumn), select areas with a water body specific gravity between 1.005-1.012, and use 120 mesh A trawl or a dredging net made of sieve silk is used to harvest the copepod population at the surface of the 30-50cm water body as a source of germplasm for screening;

[0026] (2) Directional screening: After cultivating the harvested copepod population at a temperature of 20±2°C,...

Embodiment 1

[0030] A method for screening, purifying and large-scale cultivation of Daphnia warmipla, the specific steps are as follows:

[0031] (1) Germplasm acquisition: In the estuary or aquaculture pond at the junction of salty and fresh water, when the water temperature is 20°C, select an area with a water body specific gravity of 1.008, use a 120 mesh sieve silk net to trawl or dredge, and place it on the surface of a 40cm water body Copepod populations were harvested as germplasm sources for screening;

[0032] (2) Directional screening: After cultivating the harvested copepod population for 6 days under the conditions of temperature 20°C, salinity 10, and light intensity 90 Lux, raise the water temperature to 24°C, lower the salinity to 8, and cultivate for 4 days. Then return to the initial cultivation conditions of temperature 20°C, salinity 10, and light intensity 90 Lux and cultivate for 6 days to complete a cycle of directional screening. After completing at least one cycle ...

Embodiment 2

[0036] With above-mentioned embodiment 1, its difference is:

[0037]In step (1), at the estuary at the junction of salty and fresh water, when the water temperature is 18°C, select an area with a water body specific gravity of 1.005, use a 120-mesh tulle net to trawl or dredge, and harvest copepods at the surface of the 30cm water body population as a source of germplasm for screening;

[0038] In step (2), cultivate the harvested copepod population at a temperature of 18°C, a salinity of 7, and a light intensity of 30 Lux for 7 days, raise the water temperature to 22°C, and lower the salinity to 7, cultivate for 5 days, and then restore Cultivate for 7 days under the initial cultivation conditions temperature of 18°C, salinity of 7, and light intensity of 30 Lux, and complete a cycle of directional screening. After culturing for 1-2 weeks under the conditions of 18°C, salinity 7, and light intensity 30Lux, a certain density of Daphnia warmipla populations can be obtained; ...

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Abstract

The invention discloses a method for screening, purifying and large-scale culturing of Daphnia warmipla, which is characterized in that copepod populations are captured from estuaries or breeding ponds at the junction of salty and fresh water as germplasm sources for screening; under set conditions, Carry out directional screening to select Daphnia warmi, and obtain a certain density of Daphnia warmi through culture; carry out large-scale continuous cultivation of Daphnia warmi under the optimized cultivation conditions of temperature 21.3°C, salinity 12.46, and light intensity 65.6Lux, and fish every 3 days After 3 weeks of cultivation, 2 / 3 of the total number of water fleas will form 90-100 individuals of water fleas per L of water. The advantage is that it can be cultivated at high density. Abundance can be directly used as a food source, and can also be used as opening bait for economic fish, shrimp, crab and other larvae.

Description

technical field [0001] The invention relates to the technical field of agricultural aquaculture, in particular to a method for screening, purifying and large-scale culturing of Daphnia warmipes. Background technique [0002] Copepods are a class of small and low-level crustaceans. They generally live in planktonic life and are widely distributed in oceans, freshwater or brackish water. They are an important part of zooplankton in waters and an important part of food webs in waters. links. Because its different species are distributed in different seasons and have high nutritional value, it is the opening bait for the larvae of many economic animals when raising seedlings. Therefore, it is necessary to carry out the research on nutritional enhancement and large-scale cultivation of copepods. Carrying out research on nutrition enhancement and large-scale cultivation of copepods can not only provide basic bait for seedling bait research, but also provide scientific basis for ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王子晖徐永健李响刘肖岑牟金婷
Owner NINGBO UNIV
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