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Method for artificially feeding Bemisia tabaci Gennadius

A kind of bemisia tabaci and artificial technology, applied in animal feed, animal feed, animal husbandry, etc., can solve the problems of quality, standard inconsistency, inability to obtain, experiment stop, etc., achieve low feeding cost, wide source of raw materials, guarantee standardized effect

Inactive Publication Date: 2010-12-29
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to mainly collect in the natural occurrence place of this worm for the experimental worm source of whitefly in the past, because quality, standard are not uniform, cause the difference of experimental result and when the unnatural breeding hazard period of this worm, then again Due to the lack of insect sources which lead to the suspension of the experiment, the study provides a feed formula and feeding method for artificially raising Bemisia tabaci, so that insects with the same biotype, age, and state can be obtained within a year. source to achieve standardization of test insects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: (preparation of basic feed liquid)

[0022] The components and the weight percentage of each component are: 5% of yeast extract and 10% of sucrose, supplemented to 100% with distilled water, stirred and dissolved to form the basic feed liquid.

Embodiment 2

[0023] Embodiment 2: (preparation 1 of complete nutrition feed liquid)

[0024] In the basic feed liquid of Example 1, add the following components by mg / ml, after stirring, adjust the pH value to 7.0 with 3Mol / L KOH solution, and filter and sterilize with a filter membrane to obtain an artificial full-nutrition feed liquid;

[0025]Added components include: ①Amino acids: alanine Ala 1.2, glycine Gly 2, glutamic acid Glu 2, arginine Arg 4, serine Ser 0.05, tyrosine Tyr 2, threonine The 1, valine Val 3.6, cysteine ​​Cys 2, aspartic acid Asp 0.5, histidine His 3, leucine Leu 4, isoleucine Iso 5, lysine Lys 1, proline Pro 3 , Methionine Met 0.05, Phenylalanine Phe 0.05, Tryptophan Tryptophan 2; ②Vitamins: Vitamin C 0.8, Thiamine 0.01, Inositol 0.1, Choline Chloride 0.05, Niacin 0.05, Calcium Pantothenate 0.08, Folic acid 0.01, pyridoxine hydrochloride 0.01, riboflavin 0.01, biotin 0.0002; ③ Inorganic salt: KH 2 PO 4 1. MgCl 2 ·6H 2 O 0.5, FeCl 3 ·6H 2 O 0.01, CaCl 2 2H ...

Embodiment 3

[0026] Embodiment 3: (preparation 2 of complete nutrition feed liquid)

[0027] In the basic feed liquid of Example 1, add the following components by mg / ml, after stirring, adjust the pH value to 7.0 with 3Mol / L KOH solution, and filter and sterilize with a filter membrane to obtain an artificial full-nutrition feed liquid;

[0028] Added components include: ①Amino acids: alanine Ala 3.1, glycine Gly 1.3, glutamic acid Glu5, arginine Arg 2.1, serine Ser 1.5, tyrosine Tyr 1, threonine The 2.5, valine Val2 , cysteine ​​Cys 1, aspartic acid Asp 3.2, histidine His 1.6, leucine Leu 2.5, isoleucine Iso 2.7, lysine Lys 2.5, proline Pro 1.5, methionine Met 1, phenylalanine Phe 1, tryptophan Try 1; ②Vitamins: vitamin C 0.5, thiamine 0.025, inositol 0.25, calcium pantothenate 0.05, choline chloride 0.1, folic acid 0.025, niacin 0.1 , pyridoxine hydrochloride 0.025, riboflavin 0.025, biotin 0.0005; ③ inorganic salt: KH 2 PO 4 2. MgCl 2 ·6H 2 O 1.25, CaCl 2 2H 2 O 0.025, CuCl 2 ...

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Abstract

The invention discloses a method for artificial feeding of tobacco whiteflies, which belongs to the technical field of insect feeding. The method provides a feed formula for the artificial feeding of the tobacco whiteflies and a method for feeding by applying the feed, and the method comprises the steps of the acquisition and disinfection of tobacco whitefly eggs, the preparation of an artificialall-nutrition feed liquid, the preparation of a feeding container, the feeding and so on. The method has the advantages that the method realizes the aim of the indoor artificial feeding of the tobacco whiteflies all the year around, can obtain various instar insects with relatively consistent biological type, instar stage, state and so on, overcome the defect that easy influence by different hostplants, biological types, growth states, instar stages and so on, caused by acquiring insect sources from natural birthplaces leads to the defect that the difference exists in experimental results, and ensure the standardization of test insects for experiments. The method can be used for experimental research on biological characteristics and a high-efficiency prevention and control technology ofthe tobacco whiteflies.

Description

technical field [0001] The invention relates to the technical field of insect feeding, in particular to a method for artificially feeding Bemisia tabaci. Background technique [0002] Bemisia tabaci (6ennadius) belongs to the order Homoptera and belongs to the family Aleyrodidae. It is native to tropical and subtropical regions and widely distributed in more than 90 countries and regions around the world. It is one of the main pests of more than 600 kinds of host plants such as cotton, vegetables and garden flowers (Liu Shusheng et al., 2005). Bemisia tabaci mainly damage the normal growth of plants by directly sucking plant juice, secreting honeydew, causing mold to pollute the leaf surface, affecting photosynthesis, spreading plant viruses, and inducing abnormal physiological responses of crops (Davidson et al., 2000). The harm caused by plant virus diseases is much more serious than other harms (Feng Lanxiang et al., 2001), and the loss of food and economic crops caused ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/18A01K67/033A23K1/16A23K20/142A23K20/163A23K20/174A23K20/20A23K20/24A23K20/26A23K50/90
Inventor 徐红星吕仲贤郑许松刘淑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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