Method for preparing novel silkie bone polypeptides

A new technology of black-bone chicken bone, which is applied in the field of preparation of new black-bone chicken bone polypeptide, can solve the problems of being difficult to decompose and damage the environment, and achieve the effects of retaining biologically active substances, easy absorption, and mild reaction conditions

Inactive Publication Date: 2015-05-20
ZHEJIANG XIAOERHEI FOOD
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, when people eat black-bone chicken, they only eat its meat, and its bones are thrown away as waste. In this way, a large amount of protein in black-bone chicken bones is discarded, which damages the environment in nature and is difficult to decompose.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method of a novel black-bone chicken bone polypeptide, comprising the steps of:

[0022] (1) Degreasing the black-bone chicken bones: steam the black-bone chicken bones in boiling water for 15 minutes, remove and drain for later use;

[0023] (2) black-bone chicken bone crushing: the black-bone chicken bone processed in step (1) is pulverized by a pulverizer, and sieved with 60 orders;

[0024] (3) Enzymolysis of black-bone chicken bone: step (2) put the crushed black-bone chicken bone powder and water into the container at a ratio of 100g / L, keep the temperature of the enzymatic hydrolysis solution at 40°C, and after the temperature of the enzymatic hydrolysis solution is stable, follow the enzymatic hydrolysis Add trypsin to the volume of the solution to ensure that the concentration of trypsin in the enzymolysis solution is 2.5g / L, adjust the pH of the enzymolysis solution to 8, and adjust the pH of the enzymolysis solution to 8 every 15 minutes. After ...

Embodiment 2

[0030] Compared with embodiment one, its difference is:

[0031] Step (3) Enzymolysis of black-bone chicken bone: In step (2), put the crushed black-bone chicken bone powder and water into the container at a ratio of 150g / L, keep the temperature of the enzymatic hydrolysis solution at 50°C, and after the temperature of the enzymatic hydrolysis solution is stable, follow the enzymatic Add trypsin to the volume of the hydrolysis solution to ensure that the concentration of trypsin in the enzymolysis solution is 2.5g / L, adjust the pH of the enzymolysis solution to 8.5, and adjust the pH of the enzymolysis solution to 8.5 every 15 minutes. After 4.5 hours of enzymolysis, The enzymolysis liquid is heated to boiling, and the resulting hydrolysis degree is 15.63%.

Embodiment 3

[0033] Compared with embodiment one, its difference is:

[0034] Step (3) Enzymolysis of black-bone chicken bone: In step (2), put the crushed black-bone chicken bone powder and water into a container at a ratio of 200g / L, keep the temperature of the enzymatic hydrolysis solution at 55°C, and after the temperature of the enzymatic hydrolysis solution is stable, follow the enzymatic Add trypsin to the volume of the hydrolysis solution to ensure that the concentration of trypsin in the enzymolysis solution is 4g / L, adjust the pH of the enzymolysis solution to 9, and adjust the pH of the enzymolysis solution to 9 every 15 minutes. After 6 hours of enzymolysis, the enzyme The solution was heated to boiling, and the obtained degree of hydrolysis was 14.78%.

[0035] According to the process of Examples 1 to 3, the black-bone chicken bone polypeptide powder is mixed with red date paste, pearl powder, sucrose, and water in a weight ratio of 2:3:1:1:10, and a polypeptide that promotes...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for preparing novel silkie bone polypeptides. The method comprises the following steps: (1) cooking silkie bones in boiling water for 10-20 minutes, taking out the bones, and draining for later use; (2) grinding the silkie bones treated in the step (1) by adopting a grinder, and screening by using a 40-80-mesh sieve; (3) filling the silkie bone powder ground in the step (2) and water according to a ratio of 100-200g / L, wherein the temperature of enzymatic hydrolysate is 40-55 DEG C, adding trypsin according to the volume of the enzymatic hydrolysate after the temperature of the enzymatic hydrolysate is stabilized, ensuring that the concentration of the trypsin in the enzymatic hydrolysate is 1-4g / L, regulating the pH value of the enzymatic hydrolysate to be 8-9, regulating the pH value of the enzymatic hydrolysate to be 8-9 every 10-20 minutes, performing enzymolysis for 3-6 hours, and heating the enzymatic hydrolysate until the solution is boiling; (4) filtering the enzymatic hydrolysate obtained in the step (3), thereby obtaining the filtrate; and (5) heating and concentrating the filtrate obtained in the step (4), thereby obtaining the silkie bone polypeptides.

Description

Technical field: [0001] The invention relates to the development of a health care product, in particular to a preparation method of a novel black-bone chicken bone polypeptide. Background technique: [0002] Black-bone chicken (also known as bamboo silk chicken) is a kind of omnivorous domesticated bird. The United States calls it the smooth bantam, which is short, with a small head and short neck. After evolution and reproduction distribution, it is now found in many countries. Not only are their beaks, eyes, and feet black, but their skin, muscles, bones, and most of their internal organs are also black. Due to the different breeding environments, the characteristics of black-bone chickens are also different. There are white feathers and black bones, black feathers and black bones, black bones and black meat, white meat and black bones and so on. The color of black-bone chicken feathers also becomes more varied with the way of feeding. In addition to the original white...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/10
Inventor 赵桂芹
Owner ZHEJIANG XIAOERHEI FOOD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products