Manual ultralow-temperature sample stage for iPALM (interferometric photoactivated localization microscopy) microscope
A technology of ultra-low temperature and sample stage, which is applied in the direction of material analysis, measuring devices, instruments, etc. through optical means, and can solve the problems of not being able to use iPALM
Inactive Publication Date: 2015-05-20
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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Problems solved by technology
At present, the commercially available low-temperature sample stage and room temperature sample stage for PALM microscope are single-objective lens, which cannot be used for iPALM
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[0018] attached figure 1 And attached figure 2 The manual ultra-low temperature sample stage shown includes vacuum chamber 1, substrate 2, support 3, heat insulation sleeve 4, sealing plug 5, liquid nitrogen tube 6, cover plate 7, cold core 8, large magnetic seal 9, objective lens 10, and Y adjustment rod 11. X guide rail 12, bead 13, sample rod 14, vacuum plug 15, X adjustment rod 16, middle plate 17, top plate 18, ball 19, objective lens 20, large magnetic seal 21, cover plate 22, heat insulation leg 23, Y Slider 24, Y guide rail 25, small magnetic cover 26, bead 27, pressure rod 28, X slider 29, small magnetic cover 30, Y differential head 31, X differential head 32, pressure rod 33, magnetic ring 34 , magnet 35, magnetic ring 36, fixed ring 37, magnet 38, magnetic ring 39, screw 40, retaining ring 41, magnetic ring 42, copper mesh 43, magnetic ring 44 and small magnetic ring 45 form.
[0019] The vacuum chamber 1 is made of metal or non-metal, and is in the shape of a s...
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Abstract
The invention discloses a manual ultralow-temperature sample stage for an iPALM (interferometric photoactivated localization microscopy) microscope, relates to a device which is used for applying a low-temperature transmission electron microscope biological sample to temperature preserving and position regulating of the sample of an interference type photo-activation dye positioning microscope iPALM, and aims to solve the problems of mounting an electron microscope copper net loaded with the biological sample in a narrow space, keeping the ultralow temperature of the biological sample and finely regulating the relative positions of the sample and a microscope objective. Liquid nitrogen is adopted for cooling a copper cooling core and further conducting and cooling a sample rod and the biological sample on the copper cooling core; low temperature control is performed by adopting multiple technologies of adopting a vacuum environment, a small contact surface and a heat insulating material for blocking external heat from passing through the microscope objective and a gas gap as well as passing through a vacuum cavity and an inner supporting part to conduct to the biological sample; a magnetic fluid seal technology is adopted for allowing the axial movement and the rotation of the objective and regulating an inner micro displacement table from the outer part through a differential head; a flat plate with a crossed V-shaped slot and a rolling ball are combined to form an XY displacement table to regulate XY positions of the sample.
Description
Technical field: [0001] The invention relates to a low-temperature biological sample positioning device for an interferometric fluorescent dye photoactivated localization (iPALM) microscope. Background technique: [0002] Life science is one of the most important hot research fields at present. An important manifestation of the mystery of life is the complex structure of nanoscale biomolecular machines and their miraculous and efficient biological functions. The study of the fine structure of matter is inseparable from the microscope, but the traditional optical imaging microscope is limited by the wavelength of visible light, with a resolution of only about 200 nanometers, and can only observe the micron and submicron scale structures of matter. The electron microscope has a high magnification due to the short and variable wavelength of the electron flow. Ideally, the arrangement and combination of atoms can be observed, or the resolution can reach the sub-nanometer level....
Claims
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IPC IPC(8): G01N21/01G01N21/64
Inventor 仓怀兴徐涛罗志勇孟涛张翔贾策付彦辉
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES