Device and method for cultivating active anaerobic microorganisms in self-heating liquid state

Abstract

The invention discloses a device and a method for culturing active anaerobic microorganisms in self-heating liquid. The device comprises a plastic bottle, a bactericidal sheet, a bottle cap, a heating device and a heat preservation device, wherein the plastic bottle is filled with a culture medium, and is provided with a bottle neck; an opening in the upper end of the bottle neck is sealed by an opening sealing membrane; the bactericidal sheet is arranged on the opening sealing membrane; the bottle cap caps the opening of the plastic bottle; the edge of the lower end of the bottle cap is connected with the upper end of an opening sealing ring which sleeves the bottle neck; the lower end of the opening sealing ring is connected with an rubber ring which is arranged on the bottle neck; a hollow cylinder is arranged in the bottle cap; after the opening sealing ring is torn off and the bottle cap is screwed on the plastic bottle downwards, the opening sealing membrane can be punctured by the tail end of the hollow cylinder, and the bactericidal sheet falls into the culture medium; the heating device is a heating patch; and the heat preservation device is a heat preservation box in which the plastic bottle can be put. The self-heating device provided by the invention is easy and convenient to operate, is low in economic cost and can be used for culturing anaerobic microorganisms without the help of a sterile workbench, a constant-temperature incubator and a professional technician.

Description

Technical field [0001] The invention relates to microorganism anaerobic culture equipment, in particular to a device and method for self-heating liquid culture of active anaerobic microorganisms, belonging to the technical field of environmental protection. Background technique [0002] The anaerobic culture method is mainly used for the cultivation of anaerobic bacteria such as lactic acid bacteria. Taking lactic acid bacteria as an example, they are widely present in the intestines of humans and animals. Among the more than 400 anaerobic or aerobic microorganisms in the gastrointestinal tract, lactic acid bacteria account for about 10% , Is the main member of the normal intestinal flora. As the normal flora of human and animal intestines, lactic acid bacteria are responsible for many important physiological functions of humans and animals. They can regulate the normal flora of the gastrointestinal tract of the body, maintain the microecological balance of the intestine, and pre...

AI Technical Summary

Problems solved by technology

The liquid culture method of microorganisms is usually, the first-level seed culture is to inoculate the microorganisms into the triangle flask containing the medium on the aseptic operation table, and the second-level culture (production type) is cultivated in stainless steel fermenters, and the requirements are very high during the fermentation period. , the operation is complex

[0004] At present, there are two ways to apply and sell microorganisms in the market. One is to directly sell microbial bacterial liquid products. The bacterial liquid is produced by professional enterprises, but because the microorganisms need to be stored at low temperature, the activity maintenance time is about 3 days. If it exceeds 5 days, a large number of them will die. Due to the short storage time, such products can hardly adapt to long-distance transportation and cannot meet the needs of the market; the second is to directly sell the strains and provide them to customers for cultivation. The problem of not being able to transport long distances, but without the professional knowledge of microorganisms and corresponding equipment, the quality of the produced bacterial liquid cannot be guaranteed

Therefore, the existing culture methods and sales models have many shortcomings, which limit the commercial application of liquid active microorganisms to a certain extent.

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