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A SNP marker related to the farrowing traits of Erhualian sows and its detection method and application

A Erhualian sow and marker technology, which is applied in the field of molecular biology, can solve the problems of not being able to determine the main effect gene and its key mutation sites, and achieve important economic benefits and social value

Active Publication Date: 2017-12-12
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to the website of the International Porcine QTL Database (http: / / www.animalgenome.org / cgi-bin / QTLdb / SS / index), there is currently no location on chromosomes 10, 11 and sex chromosomes in pigs that affects the total litter size. QTLs affecting total litter size have been located on other autosomes, but most of them are QTLs located by microsatellite markers, and the confidence interval is mostly 10-20cM, so it is impossible to determine the real main effect gene and its key variation site, so it is difficult to directly apply to breeding improvement of breeding pigs

Method used

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  • A SNP marker related to the farrowing traits of Erhualian sows and its detection method and application
  • A SNP marker related to the farrowing traits of Erhualian sows and its detection method and application
  • A SNP marker related to the farrowing traits of Erhualian sows and its detection method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Source of experimental animals

[0032] Jiaoxi Erhualian pig professional cooperative in Changzhou, Jiangsu.

[0033] Calculate the breeding value of 177 Erhualian sows, the calculation model is

[0034] Y (total litter size) = parity (parity) + farm (field) + year (annual) + season (season) + age (sow farrowing age) + sire (mating boar) + permanent effect (female Pig permanent effect) + additive effect (individual additive effect) + e (residual),

[0035] These included fixed effects - parity, farm / year / season sows farrowed, covariates - age of sows farrowed, random effects - boar with mating, permanent effects - sows, individual additive genetic values. Individuals with the top 10% and bottom 10% of breeding values ​​were selected. Genomic DNA extraction

[0036] Ear tissue samples from 36 sows were collected, placed in centrifuge tubes filled with 70% alcohol, and stored in a -20°C refrigerator for later use.

[0037] Using the traditional phenol / chloroform m...

Embodiment 2

[0064] This example is the verification of the SNP site g.37502410A / C obtained in Example 1 in the population of Erhualian sows.

[0065] 1. Extraction of Genomic DNA from Erhualian sows

[0066] Ear tissue samples from 197 purebred Erhualian sows with accurate records of total litter size were collected, placed in centrifuge tubes filled with 70% alcohol, and stored in a -20°C refrigerator for later use. Genomic DNA from ear tissue was extracted by the above method, and after quality and concentration detection, the concentration was diluted to 30 ng / μL and stored at -20°C for future use.

[0067] 2. PCR amplification and sequencing of the target fragment

[0068] Using the extracted DNA as a template, carry out PCR amplification according to the designed primers: take 2.5 μL of DNA template, 1.25 μL of each primer shown in SEQ ID NO: 2 and SEQ ID NO: 3, 25 μL of PCR Mix reagent, double distilled 20 μL of water; set up the PCR amplification system: pre-denaturation at 96°C ...

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Abstract

The invention discloses a SNP marker related to the farrowing traits of Erhualian sows, a detection method and application thereof. The site of the SNP marker is the g.37502410 nucleotide site on chromosome 12 of the International Porcine Genome Version 10.2 Reference Sequence, and it is an A / C mutation, corresponding to the 630th position on SEQ ID NO: 1, so The above SNP markers were significantly correlated with the total litter size of Erhualian sows. A pair of primers for detecting the SNP marker of the present invention, the upstream primer is: SEQ ID NO: 2, and the downstream primer is: SEQ ID NO: 3. The SNP markers provided by the present invention are related to the farrowing performance of Erhualian sows. Therefore, the high-yield Erhualian sow strains can be screened by identifying the SNP markers. The resulting high-productivity Erhualian sow lines have important economic benefits and Social value.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a SNP marker related to the farrowing traits of Erhualian sows, a detection method and application thereof. Background technique [0002] The litter size of pigs is an important economic trait, and the increase of the litter size will greatly increase the supply of commercial pork and bring huge economic benefits to modern pig production. With the continuous and rapid development of my country's economy, people's living standards are gradually improving, and the demand for pork is also increasing. At the end of December 2014, the number of fertile sows in my country was about 43 million. If the sows gave birth to one more litter per litter on average, based on the calculation of two litters per sow per year, it would provide about 90 million more piglets for the entire industry each year. pig. Therefore, people are also paying more and more attention to how to improve th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 李平华贺丽春黄瑞华马翔高硕
Owner NANJING AGRICULTURAL UNIVERSITY