A kind of biological control method of rice sheath blight
A rice sheath blight and seed technology, applied in the field of biological control of rice sheath blight, can solve problems such as the decline of control effect, achieve the effects of low cost, inhibit reproduction, and reduce the infection base
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Embodiment 1
[0033] A preparation method of water hyacinth tissue extract extract, the specific steps are as follows:
[0034] Step 1: Decompose the water hyacinth into roots, stems, leaves, flowers and seeds, dry them in an oven at 40°C for 5 hours, and crush them with a plant grinder;
[0035] Second step: Soak the pulverized objects of roots, stems, leaves, flowers and seeds of water hyacinth with organic solvent respectively, and the organic solvent is n-hexane; The temperature is 40°C; the slag is removed by filtration to obtain an extract with a mass concentration of 0.05g / mL;
[0036] Step 3: Concentrate the filtered extract under reduced pressure at a temperature of 40°C to obtain water hyacinth root extract, water hyacinth stem extract, water hyacinth leaf extract, and water hyacinth flower extract respectively. extract and water hyacinth seed extract.
Embodiment 2
[0038] A preparation method of water hyacinth tissue extract extract, the specific steps are as follows:
[0039] Step 1: Decompose the water hyacinth into roots, stems, leaves, flowers and seeds, dry them in an oven at 60°C for 12 hours, and crush them with a plant grinder;
[0040]Second step: Soak the pulverized objects of roots, stems, leaves, flowers and seeds of water hyacinth with an organic solvent respectively, and the organic solvent is toluene; at 60°C; remove the slag by filtration to obtain an extract with a mass concentration of 0.10 g / mL;
[0041] Step 3: Concentrate the filtered extract under reduced pressure at a temperature of 60°C to obtain water hyacinth root extract extract, water hyacinth stem extract extract, water hyacinth leaf extract extract, water hyacinth flower extract extract and water hyacinth seed extract.
Embodiment 3
[0043] A preparation method of water hyacinth tissue extract extract, the specific steps are as follows:
[0044] Step 1: Decompose the water hyacinth into roots, stems, leaves, flowers and seeds, dry them in an oven at 50°C for 8 hours, and crush them with a plant grinder;
[0045] Second step: Soak the root, stem, leaf, flower and seed of the water hyacinth with organic solvent respectively, and the organic solvent is methanol; at 50°C; remove the slag by filtration to obtain an extract with a mass concentration of 0.08g / mL;
[0046] Step 3: Concentrate the filtered extract under reduced pressure at a temperature of 50°C to obtain water hyacinth root extract, water hyacinth stem extract, water hyacinth leaf extract, and water hyacinth flower extract respectively. extract and water hyacinth seed extract.
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